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Chlorogenic acid improves the quality of boar semen processed in Percoll

2020; Colégio Brasileiro de Patologia Animal (CBPA); Volume: 17; Issue: 1 Linguagem: Inglês

10.21451/1984-3143-ar2019-0021

1984-3143

Stênia Severo Rabelo, Carla Oliveira Resende, Thaís Preisser Pontelo, Bruna Resende Chaves, Bárbara Azevedo Pereira, William Eduardo da Silva, Juliano Vogas Peixoto, Luciano José Pereira, Márcio Gilberto Zangerônimo,

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This study aimed to evaluate if the addition of chlorogenic acid (ChA) to semen extenders improves the quality of cooled boar semen processed in Percoll. The experimental design was randomized blocks (ejaculates) in a 2×3 factorial (with or without Percoll, and three antioxidant systems: a negative control, without supplementation, a positive control - vitamin E, and ChA), totaling six treatments and 12 repetitions. ChA and vitamin E (VE) were added at 4.5 mg/ml and 400 μg/ml in extender, respectively. At 0, 48 and 72h of storage at 15ºC, 80 ml insemination doses each containing 2.0 billion sperm cells were submitted to centrifugation in Percoll. The use of Percoll impaired (P<0.01) all motility patterns but decreased (P<0.01) the number of abnormal cells at 0, 48 and 72h of storage. Both VE and ChA improved (P 0.05) in semen stored for 72h. ChA improved (P 0.05) when the semen was processed in Percoll. The antioxidants had no effect (P>0.05) on the viability and integrity of the acrosome, but ChA reduced (P 0.05) of antioxidants or Percoll on the concentration of malondialdehyde in seminal plasma. The use of Percoll had no effect (P>0.05) on the cholesterol efflux, but ChA increased (P<0.05) this parameter at 0h and reduced (P<0.05) in the semen stored for 72h not processed with Percoll. In conclusion, the addition of ChA to semen extenders improved the quality of boar semen processed or not in Percoll.