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Isolation and molecular characterization of Toxoplasma gondii from placental tissues of pregnant women who received toxoplasmosis treatment during an outbreak in southern Brazil

2020; Public Library of Science; Volume: 15; Issue: 1 Linguagem: Inglês

10.1371/journal.pone.0228442

1932-6203

Camila Encarnação Minuzzi, Luiza Pires Portella, Patrícia Bräunig, Luís Antônio Sangioni, Aline Ludwig, Luciane Silva Ramos, Liliane Souto Pacheco, Camila Ribeiro Silva, Flávia Caselli Pacheco, Ivone Andreatta Menegolla, Lourdes Bonfleur Farinha, Priscila Pauli Kist, Regina Mitsuka Breganó, Beatriz de Souza Lima Nino, Felippe Danyel Cardoso Martins, Thaís Cabral Monica, Fernanda Pinto Ferreira, Isadora Britto, Ariana Signori, Kerlei Cristina Médici, Roberta Lemos Freire, João Luís Garcia, Italmar Teodorico Navarro, Cledison Márcio Difante, Fernanda Silveira Flores Vogel,

Sex work and related issues

Toxoplasma gondii is a protozoan that has great genetic diversity and is prevalent worldwide. In 2018, an outbreak of toxoplasmosis occurred in Santa Maria, Brazil, which was considered the largest outbreak ever described in the world. This paper describes the isolation and molecular characterization of Toxoplasma gondii from the placenta of two pregnant women with acute toxoplasmosis who had live births and were receiving treatment for toxoplasmosis during the outbreak. For this, placental tissue samples from two patients underwent isolation by mice bioassay, conventional PCR and genotyping using PCR-RFLP with twelve markers. Both samples were positive in isolation in mice. The isolate was lethal to mice, suggesting high virulence. In addition, the samples were positive in conventional PCR and isolates submitted to PCR-RFLP genotyping presented an atypical genotype, which had never been described before. This research contributes to the elucidation of this great outbreak in Brazil.