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Genomic evidence of yellow fever virus in Aedes scapularis, southeastern Brazil, 2016

2020; Elsevier BV; Volume: 205; Linguagem: Inglês

10.1016/j.actatropica.2020.105390

1873-6254

Mariana Sequetin Cunha, Nuno R. Faria, Giovana Santos Caleiro, Darlan Silva Candido, Sarah C. Hill, Ingra Morales Claro, Antônio Charlys da Costa, Juliana Silva Nogueira, Adriana Yurika Maeda, Fernanda Gisele da Silva, Renato Pereira de Souza, Roberta Spínola, Rosa Maria Tubaki, Regiane Maria Tironi de Menezes, Leandro Abade, Luís Filipe Mucci, María do Carmo Sampaio Tavares Timenetsky, Esther Cerdeira Sabino,

Viral Infections and Vectors

The southeastern region of Brazil has recently experienced the largest yellow fever disease outbreak in decades. Since July 2016 epizootic events were reported in São Paulo state's north region, where 787 Culicidae were captured as part of public health surveillance efforts and tested using real-time quantitative PCR. One Aedes scapularis pool collected in November 2016 in an agriculture area in Urupês city tested positive for YFV-RNA. Using a validated multiplex PCR approach we were able to recover a complete virus genome sequence from this pool. Phylogenetic analysis of the novel strain and publicly available data indicates that the belongs to the South American genotype 1 clade circulating in Sao Paulo state and is basal to the recent outbreak clade in southeast Brazil. Our findings highlight the need of additional studies, including vector competence studies, to disentangle the role of Aedes scapularis in yellow fever transmission in the Americas.