Produção Nacional
Revisado por pares
Robustness of Serologic Investigations for Chikungunya and Mayaro Viruses following Coemergence
2020; American Society for Microbiology; Volume: 5; Issue: 1 Linguagem: Inglês
10.1128/msphere.00915-19
ISSN2379-5042
AutoresCarlo Fischer, Fernando A. Bozza, Xiomara Merino, Célia Pedroso, Edmilson Ferreira de Oliveira-Filho, Andrés Moreira‐Soto, Alvaro Schwalb, Xavier de Lamballerie, Eduardo Martins Netto, Patrı́cia T. Bozza, Manoel Sarno, Carlos Brites, Eduardo Gotuzzo, Michael Talledo, Jan Felix Drexler,
Tópico(s)Viral Infections and Outbreaks Research
ResumoGeographically overlapping transmission of Chikungunya virus (CHIKV) and Mayaro virus (MAYV) in Latin America challenges serologic diagnostics and epidemiologic surveillance, as antibodies against the antigenically related viruses can be cross-reactive, potentially causing false-positive test results. We examined whether widely used ELISAs and plaque reduction neutralization testing allow specific antibody detection in the scenario of CHIKV and MAYV coemergence. For this purpose, we used 37 patient-derived MAYV-specific sera from Peru and 64 patient-derived CHIKV-specific sera from Brazil, including longitudinally collected samples. Extensive testing of those samples revealed strong antibody cross-reactivity in ELISAs, particularly for IgM, which is commonly used for patient diagnostics. Cross-neutralization was also observed, albeit at lower frequencies. Parallel testing for both viruses and comparison of ELISA reactivities and neutralizing antibody titers significantly increased diagnostic specificity. Our data provide a convenient and practicable solution to ensure robust differentiation of CHIKV- and MAYV-specific antibodies.
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