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Efflux Pump Inhibition Controls Growth and Enhances Antifungal Susceptibility of Fusarium Solani Species Complex

2020; Future Medicine; Volume: 15; Issue: 1 Linguagem: Inglês

10.2217/fmb-2019-0186

1746-0921

Rossana de Aguiar Cordeiro, Fernando VM Portela, Lívia MG Pereira, Ana RC de Andrade, José Kleybson de Sousa, Ana LR Aguiar, Mariana LM Pergentino, Gyrliane S de Sales, Jonathas Sales de Oliveira, Delia JA Medrano, Raimunda SN Brilhante, Marcos Fábio Gadelha Rocha, Débora de Souza Collares Maia Castelo‐Branco, José Júlio Costa Sidrim,

Antifungal resistance and susceptibility

Aim: To evaluate the inhibition of efflux pumps by using promethazine (PMZ) as a strategy to control Fusarium solani species complex (FSSC). Materials & methods: The susceptibility of FSSC strains to PMZ and the interaction between PMZ and antifungals were evaluated. The efflux pump activity was confirmed by flow cytometry with rhodamine 6G. Finally, PMZ was tested against FSSC biofilms. Results: PMZ inhibited FSSC planktonic growth and showed synergism with antifungals. PMZ reduced R6G efflux and inhibited cell adhesion, impaired the development of biofilms and disrupted mature biofilms. PMZ-challenged biofilms showed increased sensitivity to amphotericin B. Conclusion: The study provides indirect evidence of the occurrence of efflux pumps in FSSC and opens a perspective for this target in the control of fusariosis.