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Rabbit Eye Irritation Test: Collaborative Study

1973; Oxford University Press; Volume: 56; Issue: 4 Linguagem: Inglês

10.1093/jaoac/56.4.905

0004-5756

Francis N. Marzulli, Dennis Ruggles,

Animal testing and alternatives

Abstract The well known Draize rabbit eye irritation test was evaluated. Ten laboratories (3 government, 3 industrial, and 4 commercial) participated and 7 liquid materials were tested. Tests were conducted 2 at a time, using 1 of 6 different materials with the same control substance (70% isopropyl alcohol), for a total of 12 tests. Test materials were selected with stringency, by employing compounds which were difficult to evaluate as they were neither strongly irritant nor innocuous. By the Draize procedure, 5 of these materials were considered irritants, and 2 nonirritants. Results showed that if all 4 criteria for eye irritation (changes in cornea and iris and conjuctival redness and chemosis) were employed, collaborators were quite consistent in separating an irritant from a nonirritant substance. The method has been adopted as official first action.