Produção Nacional
Revisado por pares
First Report of Colletotrichum musicola Causing Soybean Anthracnose in Brazil
2020; American Phytopathological Society; Volume: 104; Issue: 6 Linguagem: Inglês
10.1094/pdis-12-19-2627-pdn
ISSN1943-7692
AutoresThaís Regina Boufleur, Renata Rebellato Linhares de Castro, Flávia Rogério, Maísa Ciampi‐Guillardi, Riccardo Baroncelli, Nelson Sidnei Massola Júnior,
Tópico(s)Plant pathogens and resistance mechanisms
ResumoHomePlant DiseaseVol. 104, No. 6First Report of Colletotrichum musicola Causing Soybean Anthracnose in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Colletotrichum musicola Causing Soybean Anthracnose in BrazilT. R. Boufleur, R. R. L. Castro, F. Rogério, M. Ciampi-Guillardi, R. Baroncelli, and N. S. Massola JúniorT. R. Boufleurhttp://orcid.org/0000-0002-6357-0823Luiz de Queiroz College of Agriculture (ESALQ), University of São Paulo (USP), Department of Plant Pathology and Nematology, Piracicaba, São Paulo, 13418-900, Brazil, R. R. L. Castrohttp://orcid.org/0000-0001-9488-7164Luiz de Queiroz College of Agriculture (ESALQ), University of São Paulo (USP), Department of Plant Pathology and Nematology, Piracicaba, São Paulo, 13418-900, Brazil, F. RogérioLuiz de Queiroz College of Agriculture (ESALQ), University of São Paulo (USP), Department of Plant Pathology and Nematology, Piracicaba, São Paulo, 13418-900, Brazil, M. Ciampi-Guillardihttp://orcid.org/0000-0002-5175-4860Luiz de Queiroz College of Agriculture (ESALQ), University of São Paulo (USP), Department of Plant Pathology and Nematology, Piracicaba, São Paulo, 13418-900, Brazil, R. Baroncellihttp://orcid.org/0000-0002-5878-1159Instituto Hispano-Luso de Investigaciones Agrarias (CIALE), Universidad de Salamanca, Department of Microbiology and Genetics, Villamayor, Salamanca, 37185, Spain, and N. S. Massola Júnior†Corresponding author: N. S. Massola Júnior; E-mail Address: [email protected]Luiz de Queiroz College of Agriculture (ESALQ), University of São Paulo (USP), Department of Plant Pathology and Nematology, Piracicaba, São Paulo, 13418-900, BrazilAffiliationsAuthors and Affiliations T. R. Boufleur1 R. R. L. Castro1 F. Rogério1 M. Ciampi-Guillardi1 R. Baroncelli2 N. S. Massola Júnior1 † 1Luiz de Queiroz College of Agriculture (ESALQ), University of São Paulo (USP), Department of Plant Pathology and Nematology, Piracicaba, São Paulo, 13418-900, Brazil 2Instituto Hispano-Luso de Investigaciones Agrarias (CIALE), Universidad de Salamanca, Department of Microbiology and Genetics, Villamayor, Salamanca, 37185, Spain Published Online:17 Apr 2020https://doi.org/10.1094/PDIS-12-19-2627-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Soybean (Glycine max L.) is one of the most important crops worldwide as a source of protein-rich foods and animal feeds. Anthracnose, one of the major limiting factors to soybean production (Dias et al. 2016), is caused by species such as Colletotrichum truncatum, C. sojae, and C. plurivorum (Damm et al. 2009, 2019). In December 2016 and 2017, soybean plants of cultivars Monsoy 8768 and Pioneer y-70 with typical symptoms of anthracnose (necrotic and irregular brown lesions on stems, leaves, and pods) were collected in Mato Grosso, Brazil. Commercial fields sampled showed 10 to 15% incidence of anthracnose in 1 ha in each sampled area. In total, 10 different geographic locations were sampled. Colletotrichum strains were isolated and cultured on potato dextrose agar at 25°C with a 12-h light photoperiod from surface-disinfected (70% alcohol followed by 0.5% sodium hypochlorite) plant tissues. Among others, three single-spore isolates (LFN0048 from Sinop, LFN0074 and LFN0090 from Lucas do Rio Verde) showed different morphology; isolates LFN0048 and LFN0074 were selected for further characterization. Total DNA was extracted and partial glyceraldehyde 3-phosphate dehydrogenase (GAPDH), histone H3 (HIS3), and β-tubulin (TUB2) genes were amplified and sequenced. The sequences were deposited in GenBank (accession numbers MN604249 and MK163893 for HIS3, MN604248 and MK142674 for GAPDH, and MN604250 and MK142675 for TUB) and were compared with most similar reference sequences of Colletotrichum (Damm et al. 2019). Both isolates clustered with Colletotrichum musicola epitype (CBS 132885), showing 100 and 98.5% similarity in GAPDH, 99.5 and 98.9% in HIS3, and 99.2% in TUB2. On PDA, colonies showed dark-gray aerial mycelium with entire margins, reverse violaceous-black. Conidia and ascospore size and shape match those previously described by Damm et al. (2019): 12.12 to 15.86 × 4.93 to 6.95 µm and 15.5 to 19.34 × 5 to 7.84 µm, respectively (n = 100). Appressoria (n = 50) were single or in loose groups, violaceous-black with predominant obovoid, truncated, and cylindrical shapes, with smooth, undulate, or lobate margin, and 9.25 to 29.79 × 7.22 to 21.06 µm. Perithecia, paraphyses; and unitunicate eight-spored asci were also observed. Asci were cylindrical to clavate, smooth-walled, and 48.12 to 68.78 × 9.59 to 14.47 µm (n = 50). Soybean anthracnose is seed-borne (Dias et al. 2018; Rogério et al. 2017); therefore, pathogenicity tests were carried out on pregerminated seeds. Five seeds of Brasmax 8579 cultivar were inoculated with 10 µl of a conidial suspension (106 conidia/ml) that was placed in the emerging radicle, and five mock-inoculated seeds were used as a control. Seedlings were planted in vermiculite and incubated at 25°C with a 12-h photoperiod. After 7 days, inoculated plants showed necrotic lesions on the cotyledons, leaflets, and hypocotyl, whereas control plants remained asymptomatic. The experiment was repeated three times. C. musicola was reisolated from the symptomatic tissues, and the identity was confirmed by morphology and multilocus phylogeny. Until now, C. musicola has been reported to be associated with Musa sp. (Damm et al. 2019) and Colocasia esculenta (Vásquez-López et al. 2019) in Mexico, and with Phaseolus lunatus in Brazil (Cavalcante et al. 2018). To our knowledge, this is the first report of C. musicola joining a group of new and emergent species of Colletotrichum causing anthracnose in soybean-producing regions around the world.The author(s) declare no conflict of interest.References:Cavalcante, G. R. S., et al. 2018. Plant Dis. 102:680. https://doi.org/10.1094/PDIS-07-17-0963-PDN Link, ISI, Google ScholarDamm, U., et al. 2009. Fung. Div. 39:45. ISI, Google ScholarDamm, U., et al. 2019. Stud. Mycol. 92:1. https://doi.org/10.1016/j.simyco.2018.04.001 Crossref, ISI, Google ScholarDias, M., et al. 2018. Trop. Plant Pathol. 43:439. https://doi.org/10.1007/s40858-018-0249-6 Crossref, ISI, Google ScholarDias, M. P., et al. 2016. Summa Phytopathol. 42:18. https://doi.org/10.1590/0100-5405/2114 Crossref, Google ScholarRogério, F. et al. 2017. J. Appl. Microbiol. 122:402https://doi.org/10.1111/jam.13346. Google ScholarVásquez-López, A., et al. 2019. Plant Dis. 103:2963. https://doi.org/10.1094/PDIS-05-19-0967-PDN Link, ISI, Google ScholarT. R. Boufleur and R. R. L. Castro contributed equally to this manuscript.The author(s) declare no conflict of interest.Funding: The authors thank Fundação de Amparo à Pesquisa do Estado de São Paulo (2017/09178-8) and CNPq (70328/1997-2) for financial support. Research of R. Baroncelli is supported by the project Escalera de Excelencia CLU-2018-04 co-funded by the P.O. FEDER of Castilla y León 2014-2020 Spain.DetailsFiguresLiterature CitedRelated Vol. 104, No. 6 June 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionSymptoms of citrus yellow mottle-associated virus on a leaf of Washington navel orange (J. X. Wu et al.). Photo credit: M. J. Cao. Effect of pydiflumetofen + difenoconazole on the severity of Cercospora leaf spot caused by Cercospora beticola in a small plot (S. J. Pethybridge et al.). Photo credit: S. J. Pethybridge. Metrics Article History Issue Date: 8 Jun 2020Published: 17 Apr 2020First Look: 16 Feb 2020Accepted: 11 Feb 2020 Page: 1858 Information© 2020 The American Phytopathological SocietyFundingFundação de Amparo à Pesquisa do Estado de São PauloGrant/Award Number: 2017/09178-8KeywordsGlycine maxGlomerellalegumesfungal diseaseThe author(s) declare no conflict of interest.Cited bySpecies diversity, resistance to MBC fungicides, and low sensitivity to azoxystrobin in field isolates of Colletotrichum spp. associated with soybean anthracnose in Mato Grosso and Goiás States, Brazil22 December 2022 | Tropical Plant Pathology, Vol. 68Comparative transcriptomic provides novel insights into the soybean response to Colletotrichum truncatum infection25 November 2022 | Frontiers in Plant Science, Vol. 13Pest categorisation of Colletotrichum aenigma, C. alienum, C. perseae, C. siamense and C. theobromicolaEFSA Journal, Vol. 20, No. 8Colletotrichum pereskiae sp. nov. causing anthracnose on Pereskia aculeata in Brazil2 December 2021 | Mycological Progress, Vol. 20, No. 12An Analysis of Postharvest Fungal Pathogens Reveals Temporal–Spatial and Host–Pathogen Associations with Fungicide Resistance-Related MutationsMichael J. Bradshaw, Holly P. Bartholomew, Dylan Hendricks, Autumn Maust, and Wayne M. Jurick14 November 2021 | Phytopathology®, Vol. 111, No. 11Identification and Comparison of Colletotrichum Secreted Effector Candidates Reveal Two Independent Lineages Pathogenic to Soybean21 November 2021 | Pathogens, Vol. 10, No. 11Pest categorisation of Colletotrichum plurivorumEFSA Journal, Vol. 19, No. 11Soybean anthracnose caused by Colletotrichum species: Current status and future prospects20 February 2021 | Molecular Plant Pathology, Vol. 22, No. 4Genome Sequence Resources of Colletotrichum truncatum, C. plurivorum, C. musicola, and C. sojae: Four Species Pathogenic to Soybean (Glycine max)Flávia Rogério, Thaís R. Boufleur, Maisa Ciampi-Guillardi, Serenella A. Sukno, Michael R. Thon, Nelson Sidnei Massola Júnior, and Riccardo Baroncelli7 July 2020 | Phytopathology®, Vol. 110, No. 9
Referência(s)