First Report of Leaf Anthracnose Caused by Colletotrichum tropicale on Oregano ( Origanum vulgare ) in Mexico
2020; American Phytopathological Society; Volume: 104; Issue: 6 Linguagem: Inglês
10.1094/pdis-01-20-0169-pdn
ISSN1943-7692
AutoresSergio Ayvar-Serna, José Francisco Díaz-Nájera, Antonio Mena-Bahena, B. E. Ortiz-Montes, Omar G. Alvarado-Gómez, Nelson Bernardi Lima, Juan Manuel Tovar‐Pedraza,
Tópico(s)Mycorrhizal Fungi and Plant Interactions
ResumoHomePlant DiseaseVol. 104, No. 6First Report of Leaf Anthracnose Caused by Colletotrichum tropicale on Oregano (Origanum vulgare) in Mexico PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Leaf Anthracnose Caused by Colletotrichum tropicale on Oregano (Origanum vulgare) in MexicoS. Ayvar-Serna, J. F. Díaz-Nájera, A. Mena-Bahena, B. E. Ortiz-Montes, O. G. Alvarado-Gómez, N. B. Lima, and J. M. Tovar-PedrazaS. Ayvar-SernaColegio Superior Agropecuario del Estado de Guerrero, Departamento de Fitotecnia, Iguala, 40000, Guerrero, Mexico, J. F. Díaz-NájeraColegio Superior Agropecuario del Estado de Guerrero, Departamento de Fitotecnia, Iguala, 40000, Guerrero, Mexico, A. Mena-BahenaColegio Superior Agropecuario del Estado de Guerrero, Departamento de Fitotecnia, Iguala, 40000, Guerrero, Mexico, B. E. Ortiz-MontesColegio Superior Agropecuario del Estado de Guerrero, Departamento de Fitotecnia, Iguala, 40000, Guerrero, Mexico, O. G. Alvarado-GómezUniversidad Autónoma de Nuevo León, Facultad de Agronomía, San Nicolás de los Garza, 66455, Nuevo León, Mexico, N. B. Limahttp://orcid.org/0000-0002-6145-5119Instituto de Patología Vegetal (CIAP-INTA), Unidad de Fitopatología y Modelización Agrícola (UFYMA), CONICET. Córdoba, X5020, Argentina, and J. M. Tovar-Pedraza†Corresponding author: J. M. Tovar-Pedraza; E-mail Address: juan.tovar@ciad.mxhttp://orcid.org/0000-0002-8560-959XCentro de Investigación en Alimentación y Desarrollo, Coordinación Culiacán, Laboratorio de Fitopatología, Culiacán, 80110, Sinaloa, Mexico AffiliationsAuthors and Affiliations S. Ayvar-Serna1 J. F. Díaz-Nájera1 A. Mena-Bahena1 B. E. Ortiz-Montes1 O. G. Alvarado-Gómez2 N. B. Lima3 J. M. Tovar-Pedraza4 † 1Colegio Superior Agropecuario del Estado de Guerrero, Departamento de Fitotecnia, Iguala, 40000, Guerrero, Mexico 2Universidad Autónoma de Nuevo León, Facultad de Agronomía, San Nicolás de los Garza, 66455, Nuevo León, Mexico 3Instituto de Patología Vegetal (CIAP-INTA), Unidad de Fitopatología y Modelización Agrícola (UFYMA), CONICET. Córdoba, X5020, Argentina 4Centro de Investigación en Alimentación y Desarrollo, Coordinación Culiacán, Laboratorio de Fitopatología, Culiacán, 80110, Sinaloa, Mexico Published Online:17 Apr 2020https://doi.org/10.1094/PDIS-01-20-0169-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Oregano (Origanum vulgare L.) is a perennial herb belonging to the Lamiaceae family and is commonly grown for its aromatic and medicinal properties. In January 2019, symptoms of leaf anthracnose were observed on approximately 25% of oregano plants located in a nursery in Huitzuco de los Figueroa, Guerrero, Mexico. Lesions on oregano leaves were irregular, brown, and yellow-haloed. For fungal isolation, small pieces from tissue at the margins of lesions of symptomatic leaves were surface disinfested by immersion in a 1% sodium hypochlorite solution for 2 min, rinsed in sterile distilled water, and placed onto potato dextrose agar (PDA) in Petri plates. The plates were incubated at 25°C for 5 days in darkness. Colletotrichum-like colonies were consistently obtained, and five monoconidial isolates were made. Only a single morphotype was observed, and an isolate was randomly selected for characterization, multilocus phylogenetic analysis, and pathogenicity tests. The isolate was deposited in the Culture Collection of Phytopathogenic Fungi at the Colegio Superior Agropecuario del Estado de Guerrero (accession no. CSAEG-CO19). After 8 days on PDA, the colonies were white to gray on the upper surface and had orange conidial masses. Conidia (n = 100) were cylindrical, hyaline, aseptate, with rounded ends, and measured 13.6 to 17.8 × 3.9 to 5.4 μm. Based on morphological features, the isolate was tentatively identified as a member of the Colletotrichum gloeosporioides species complex (Weir et al. 2012). Total DNA was extracted, and the internal transcribed spacer (ITS) region (White et al. 1990) and partial sequences of actin (ACT) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes were amplified by polymerase chain reaction (PCR) (Weir et al. 2012) for sequencing and molecular identification. A phylogenetic tree based on Bayesian inference and including published ITS, GAPDH, and ACT data for Colletotrichum species was constructed. The multilocus phylogenetic analysis clearly distinguished the isolate CSAEG-CO19 as Colletotrichum tropicale, separating it from all other species within the C. gloeosporioides species complex. DNA sequences were deposited in GenBank (accession nos.: ITS, MN947334; GAPDH, MN942958; and ACT, MN942957). Pathogenicity of the fungus was verified by spraying a conidial suspension (1 × 105 spores/ml) on 10 leaves on each of 10 healthy oregano plants. Ten control plants were sprayed with sterile distilled water. All inoculated plants and controls were covered with a plastic bag for 24 h and then were kept under greenhouse conditions at 25 to 30°C for 18 days. Anthracnose symptoms were observed on all inoculated leaves after 15 days, whereas control leaves remained healthy. The fungus was consistently reisolated from leaf lesions, fulfilling Koch's postulates. To date, only Colletotrichum fioriniae in the Colletotrichum acutatum species complex has been reported as a leaf pathogen on oregano, in Italy (Guarnaccia et al. 2019). However, to our knowledge, this is the first report of C. tropicale causing leaf anthracnose on oregano worldwide.The author(s) declare no conflict of interest.References:Guarnaccia, V., et al. 2019. Agronomy (Basel) 9:613. https://doi.org/10.3390/agronomy9100613 Crossref, ISI, Google ScholarWeir, B. S., et al. 2012. Stud. Mycol. 73:115. https://doi.org/10.3114/sim0011 Crossref, ISI, Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA. Crossref, Google ScholarThe author(s) declare no conflict of interest.Funding: This research was partially supported by the National Council of Science and Technology of Mexico (CONACYT), project INFRA–2019–302117.DetailsFiguresLiterature CitedRelated Vol. 104, No. 6 June 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionSymptoms of citrus yellow mottle-associated virus on a leaf of Washington navel orange (J. X. Wu et al.). Photo credit: M. J. Cao. Effect of pydiflumetofen + difenoconazole on the severity of Cercospora leaf spot caused by Cercospora beticola in a small plot (S. J. Pethybridge et al.). Photo credit: S. J. Pethybridge. Metrics Downloaded 836 times Article History Issue Date: 8 Jun 2020Published: 17 Apr 2020First Look: 26 Feb 2020Accepted: 20 Feb 2020 Pages: 1855-1855 Information© 2020 The American Phytopathological SocietyKeywordsfungipathogen detectionColletotrichumThe author(s) declare no conflict of interest.Cited ByImportant Fungal Diseases in Medicinal and Aromatic Plants and Their ControlTurkish Journal of Agricultural Engineering Research, Vol. 2, No. 1
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