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Mesenchymal Stromal Cells Derived from Bone Marrow and Adipose Tissue: Isolation, Culture, Characterization and Differentiation

2020; American Academy of Arts and Sciences; Volume: 10; Issue: 4 Linguagem: Inglês

10.21769/bioprotoc.3534

2331-8325

Gileade Pereira Freitas, Alann Thaffarell Portilho Souza, Helena Bacha Lopes, Rayana Longo Bighetti‐Trevisan, Fabíola Cristina Ribeiro de Oliveira, Roger Rodrigo Fernandes, Fernanda Silva Ferreira, Felipe Augusto Rós, Márcio Mateus Beloti, Adalberto Luiz Rosa,

Bone fractures and treatments

Since their discovery, mesenchymal stromal cells (MSCs) have received a lot of attention, mainly due to their self-renewal potential and multilineage differentiation capacity. For these reasons, MSCs are a useful tool in cell biology and regenerative medicine. In this article, we describe protocols to isolate MSCs from bone marrow (BM-MSCs) and adipose tissues (AT-MSCs), and methods to culture, characterize, and differentiate MSCs into osteoblasts, adipocytes, and chondrocytes. After the harvesting of cells from bone marrow by flushing the femoral diaphysis and enzymatic digestion of abdominal and inguinal adipose tissues, MSCs are selected by their adherence to the plastic tissue culture dish. Within 7 days, MSCs reach 70% confluence and are ready to be used in subsequent experiments. The protocols described here are easy to perform, cost-efficient, require minimal time, and yield a cell population rich in MSCs.