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Transmission of Anaplasma marginale by unfed Rhipicephalus microplus tick larvae under experimental conditions

2020; National Institute of Research for Forestry, Agricultural and Livestock; Volume: 11; Issue: 1 Linguagem: Inglês

10.22319/rmcp.v11i1.5018

2448-6698

Itzel Amaro-Estrada, Miguel A. García-Ortíz, Jesús Francisco Preciado de la Torre, Edmundo E. Rojas-Ramírez, Rubén Hernández-Ortíz, Francisco Alpírez-Mendoza, Sergio D. Rodríguez Camarillo,

Insect and Pesticide Research

The current description of biological transmission of Anaplasma marginale by Rhipicephalus microplus ticks, includes both intrastadial and transstadial transmission. Both transovarial transmission of Anaplasma from engorged ticks to their progeny and, transmission from infected unfed larvae to the mammalian host are is controversial. In order to demonstrate vertical transmission of A. marginale by R. microplus ticks under experimental conditions, feed-acquisition infected engorged females were incubated at 18° C or 28° C for oviposition. Larvae hatched from these ticks were used to infest two steers for each incubation temperature. None of the four steers infested with either lot of larvae developed clinical disease, yet subclinical infection was observed in the steers infested with larvae from engorged ticks incubated at 28°. DNA from larvae used for the infection of the carrier tick donor, Only DNA from larvae oviposited at 28 ºC, DNA from blood of positive steers were positive for amplification of msp5 and msp1α variable region by PCR. All other DNA samples from the original stabilate, blood from the donor steer, larvae from ticks incubated at 28° C and steers infested with these same larvae were positive both to msp5 and msp1α PCR. msp1α sequences of all PCR products were the same and coincided with previously reported Tlapacoyan-2 sequence. The present evidence indicates that R. microplus is capable of transmitting A. marginale to its progeny and that these infected larvae can transmit the infection to susceptible hosts.