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Comparison of antibody repertories against Staphylococcus aureus in healthy and infected dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine

2020; Elsevier BV; Volume: 103; Issue: 5 Linguagem: Inglês

10.3168/jds.2019-17084

1529-9066

Adriano França da Cunha, Hélida Monteiro de Andrade, Fernando N. Souza, L.C. Fialho, Dalila Lapinha Silva Oliveira Rosa, Eduardo Milton Ramos-Sanchez, Magnus Gidlund, Hiro Goto, Maria Aparecida Vasconcelos Paiva Brito, Alessandro de Sá Guimarães, Andrey Pereira Lage, Luiza Campos Reis, Alice Maria Melville Paiva Della Libera, Marcos Bryan Heinemann, M.M.O.P. Cerqueira,

Microbial infections and disease research

ABSTRACT Staphylococcus aureus is one of the pathogens most frequently isolated from cases of mastitis worldwide. To decrease the effect of S. aureus mastitis in dairy farming, alternative strategies for controlling mastitis are needed that depend on a better knowledge of cow-to-cow variations in S. aureus antibody production. The present study sought to explore the diversity of S. aureus antibodies produced by dairy cows with a distinct mastitis history and vaccinated with a polyvalent mastitis vaccine. We obtained protein extracts from S. aureus isolates derived from persistent subclinical mastitis. Proteins were fractionated using 2-dimensional gel electrophoresis and Western blotting. Then, Western blotting membranes were exposed to sera from 24 dairy cows that had been divided into the following groups: vaccinated dairy cows that were infected with S. aureus , further subdivided according to whether they (a) remained infected by S. aureus or (b) recovered from the intramammary infection; unvaccinated dairy cows infected with S. aureus ; and vaccinated healthy dairy cows with no history of S. aureus mastitis. Proteins found to be reactive by Western blot were identified by mass spectrometry (MALDI/TOF-TOF). Our most important finding was that F 0 F 1 ATP synthase subunit α, succinyl-diaminopimelate desuccinylase, and cysteinyl-tRNA synthetase were potential candidate proteins for the prevention of S. aureus mastitis. This study strengthens the notion that variations among animals should not be ignored and shows that the heterogeneity of antibody production against anti-staphylococcal antigens in animals may enable the identification of new immunotherapy targets.