Portal de Periódicos da CAPES

An oligosaccharide-marker approach to quantify specific polysaccharides in herbal formula by LC-qTOF-MS: Danggui Buxue Tang, a case study

2020; Elsevier BV; Volume: 185; Linguagem: Inglês

10.1016/j.jpba.2020.113235

1873-264X

Lifeng Li, Tin-Long Wong, Jixia Zhang, Lishuang Zhou, Su‐Ping Bai, Hau-Yee Fung, Huiyuan Cheng, Quanwei Zhang, Hongming Zheng, Wan-Rong Bao, Dik‐Lung Ma, Chung‐Hang Leung, Ge Zhang, Zhaoxiang Bian, Aiping Lyu, Quan‐Bin Han,

Traditional Chinese Medicine Analysis

Polysaccharides have broad bioactivities and are major components of water decoction of herb formulae. However, the quality control of polysaccharides remains a challenge. Oligosaccharide-fragment approach has been considered in elucidating chemical structures of polysaccharides, but never been used for quantitation. Using reference chemicals and a real sample Danggui Buxue Tang (DBT) in this study, an oligosaccharide-marker approach was established to quantify specific polysaccharides. Firstly, linear relationships between parent polysaccharides and hydrolysis-produced daughter oligosaccharides were verified using reference polysaccharides. Then in case of DBT, two fluorescence-labeled oligosaccharides with high specificity to individual parent polysaccharides were selected as markers. They were easily isolated and identified. Their potential in quantification of parent polysaccharides were satisfactorily validated in terms of linearity (r≥0.99), repeatability (RSD ≤ 8.4 %), and spike recovery (≥80 %). This method could be a promising approach for quality assessment of polysaccharides in herbal formulae.