Produção Nacional
Revisado por pares
Occurrence of Atypical and New Genotypes of Toxoplasma Gondii in Free-Range Chickens Intended for Human Consumption in Brazil
2020; Springer Science+Business Media; Volume: 65; Issue: 3 Linguagem: Inglês
10.2478/s11686-020-00194-2
ISSN1896-1851
AutoresAna Clécia dos Santos Silva, Luiz Daniel de Barros, Viviane Melo Coelho Barros, Adrianne Mota de Alcântara, Müller Ribeiro-Andrade, João Luís Garcia, Rinaldo Aparecido Mota, Wagnner José Nascimento Porto,
Tópico(s)Herpesvirus Infections and Treatments
ResumoThe aim of the present study was to detect and genotype T. gondii in free-range chickens destined to human consumption in Alagoas state, Brazil.Two hundred blood samples were collected from free-range chickens and submitted to indirect immunofluorescence antibody test (IFAT). Brain tissue from 14 animals randomly selected were subjected to mouse bioassay. Positive samples in mouse bioassay were submitted to PCR and genotyped by PCR-RFLP.Out of two hundred blood samples from chickens, 72 (36%) samples were considered positive by IFAT. Two T. gondii strains were isolated, both being characterized as atypical and classified as #146 and a new genotype, named #279 in ToxoDB database.Our results showed a sero-occurrence of T. gondii in free-range chickens intended for humans, and the genetic diversity of the parasite in Brazil, with a new genotype described.
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