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Multi-lineage Human iPSC-Derived Platforms for Disease Modeling and Drug Discovery

2020; Elsevier BV; Volume: 26; Issue: 3 Linguagem: Inglês

10.1016/j.stem.2020.02.011

1934-5909

Arun Sharma, Samuel Sances, Michael J. Workman, Clive N. Svendsen,

Tissue Engineering and Regenerative Medicine

Human induced pluripotent stem cells (hiPSCs) provide a powerful platform for disease modeling and have unlocked new possibilities for understanding the mechanisms governing human biology, physiology, and genetics. However, hiPSC-derivatives have traditionally been utilized in two-dimensional monocultures, in contrast to the multi-systemic interactions that influence cells in the body. We will discuss recent advances in generating more complex hiPSC-based systems using three-dimensional organoids, tissue-engineering, microfluidic organ-chips, and humanized animal systems. While hiPSC differentiation still requires optimization, these next-generation multi-lineage technologies can augment the biomedical researcher's toolkit and enable more realistic models of human tissue function.