Origins and clonal convergence of gastrointestinal IgE + B cells in human peanut allergy
2020; American Association for the Advancement of Science; Volume: 5; Issue: 45 Linguagem: Inglês
10.1126/sciimmunol.aay4209
ISSN2470-9468
AutoresRamona A. Hoh, Shilpa A. Joshi, Ji-Yeun Lee, Brock A. Martin, Sushama Varma, Shirley Kwok, Sandra C. A. Nielsen, Parastu Nejad, Emily Haraguchi, Priya S. Dixit, Swetha V. Shutthanandan, Krishna M. Roskin, Wenming Zhang, Dana Tupa, Bryan Bunning, Monali Manohar, Robert Tibshirani, Nielsen Fernandez‐Becker, Neeraja Kambham, Robert B. West, Robert G. Hamilton, Mindy Tsai, Stephen J. Galli, R. Sharon Chinthrajah, Kari C. Nadeau, Scott D. Boyd,
Tópico(s)Eosinophilic Esophagitis
ResumoB cells in human food allergy have been studied predominantly in the blood. Little is known about IgE+ B cells or plasma cells in tissues exposed to dietary antigens. We characterized IgE+ clones in blood, stomach, duodenum, and esophagus of 19 peanut-allergic patients, using high-throughput DNA sequencing. IgE+ cells in allergic patients are enriched in stomach and duodenum, and have a plasma cell phenotype. Clonally related IgE+ and non-IgE-expressing cell frequencies in tissues suggest local isotype switching, including transitions between IgA and IgE isotypes. Highly similar antibody sequences specific for peanut allergen Ara h 2 are shared between patients, indicating that common immunoglobulin genetic rearrangements may contribute to pathogenesis. These data define the gastrointestinal tract as a reservoir of IgE+ B lineage cells in food allergy.
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