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Simultaneous fluorescence lifetime and Raman fiber-based mapping of tissues

2020; Optica Publishing Group; Volume: 45; Issue: 8 Linguagem: Inglês

10.1364/ol.389300

1539-4794

João L. Lagarto, Vladislav I. Shcheslavskiy, Francesco S. Pavone, Riccardo Cicchi,

Optical Imaging and Spectroscopy Techniques

We report the development of a novel, to the best of our knowledge, fiber-based system to realize coregistered simultaneous acquisition of fluorescence lifetime (FL) data and Raman spectra from the same area. FL measurements by means of time-correlated single photon counting are realized with periodic out-of-phase external illumination of the field of view, enabling acquisition of data under bright illumination of the specimen. Raman measurements in the near-infrared are realized asynchronously. We present a detailed characterization of this technique and validate its potential to report intrinsic contrast. Fiber-based FL and Raman maps report complementary structural, compositional, and molecular contrast in biological tissues with diverse compositional features.