First Report of Duranta leaf curl virus Infecting Ficus virens Showing Leaf Curl Symptoms in Pakistan
2020; American Phytopathological Society; Volume: 104; Issue: 7 Linguagem: Inglês
10.1094/pdis-12-19-2719-pdn
ISSN1943-7692
AutoresAamir Lal, Eui‐Joon Kil, Võ Thị Bích Thuỷ, Chairina Fadhila, Phuong T. Ho, Malik Nawaz Shuja, Muhammad Ali, Sukchan Lee,
Tópico(s)Insect-Plant Interactions and Control
ResumoHomePlant DiseaseVol. 104, No. 7First Report of Duranta leaf curl virus Infecting Ficus virens Showing Leaf Curl Symptoms in Pakistan PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Duranta leaf curl virus Infecting Ficus virens Showing Leaf Curl Symptoms in PakistanAamir Lal, Eui-Joon Kil, Thuy Thi Bich Vo, Chairina Fadhila, Phuong Thi Ho, Malik Nawaz Shuja, Muhammad Ali, and Sukchan LeeAamir LalDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, KoreaSearch for more papers by this author, Eui-Joon Kil†Corresponding authors: E.-J. Kil; E-mail Address: meitantei007@naver.com and S. C. Lee; E-mail Address: cell4u@skku.eduhttp://orcid.org/0000-0002-7256-3879Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, KoreaDepartment of Plant Medicals, Andong National University, Andong 36729, KoreaSearch for more papers by this author, Thuy Thi Bich VoDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, KoreaSearch for more papers by this author, Chairina FadhilaDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, KoreaSearch for more papers by this author, Phuong Thi HoDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, KoreaSearch for more papers by this author, Malik Nawaz Shujahttp://orcid.org/0000-0002-8255-7675Department of Microbiology, Kohat University of Science and Technology, Kohat 26000, PakistanSearch for more papers by this author, Muhammad AliDepartment of Life Sciences, School of Science, University of Management and Technology, Lahore 54770, PakistanSearch for more papers by this author, and Sukchan Lee†Corresponding authors: E.-J. Kil; E-mail Address: meitantei007@naver.com and S. C. Lee; E-mail Address: cell4u@skku.eduDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, KoreaSearch for more papers by this authorAffiliationsAuthors and Affiliations Aamir Lal1 Eui-Joon Kil1 2 † Thuy Thi Bich Vo1 Chairina Fadhila1 Phuong Thi Ho1 Malik Nawaz Shuja3 Muhammad Ali4 Sukchan Lee1 † 1Department of Integrative Biotechnology, Sungkyunkwan University, Suwon 16419, Korea 2Department of Plant Medicals, Andong National University, Andong 36729, Korea 3Department of Microbiology, Kohat University of Science and Technology, Kohat 26000, Pakistan 4Department of Life Sciences, School of Science, University of Management and Technology, Lahore 54770, Pakistan Published Online:12 May 2020https://doi.org/10.1094/PDIS-12-19-2719-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Ficus virens (family: Moraceae) is a deciduous tree found in the Indian subcontinent, some other Southeast Asian countries, and northern Australia. It is valued for its latex, wood, and medicinal and edible products. In December 2018, during a routine survey to record begomoviruses other than cotton infecting viruses in Pakistan, curling of young leaves, which is a typical symptom of infection by viruses of the genus Begomovirus, was observed in F. virens trees in a park in Lahore, central Punjab, Pakistan. The total DNA of three trees was extracted using the Viral Gene-spin Viral DNA/RNA Extraction Kit (iNtRON Biotechnology, Seongnam, Korea) and used as a template in PCR amplification with begomovirus-specific primers: Beg-F (5′-CCGTGCTGCTGCCCCCATTGTCCGCGTCAC-3′) and Beg-R (5′- CTGCCACAACCATGGATTCACGCACAGGG-3′). The amplified 1-kb DNA was cloned into the pGEM-3Zf (+) vector (Promega, Madison, WI) and sequenced by a commercial sequencing service (Macrogen, Seoul, Korea). NCBI BLAST results revealed a nucleotide (nt) sequence identity of 95, 93, and 92% with chilli leaf curl India virus, rose leaf curl virus, and papaya leaf crumple virus, respectively. Based on the sequence obtained, new primers, DCP-F (5′-AGCACATTGGTAAAGTCATGTGTG-3′) and DCP-R (5′-GGATATCATGTCTGGACTCAAATG-3′), were designed to obtain full-length DNA-A of the begomovirus. An approximately 2.8-kb product was amplified and cloned. The resulting sequence (GenBank accession no. MN537564) showed 97.43% nt identity with duranta leaf curl virus (DLCV; KT948069), a Begomovirus species reported in the ornamental shrub Duranta erecta in Pakistan by Iram et al. (2005) and by Anwar et al. (2017). In the former case, only DNA-A of DLCV was found without any associated molecules such as DNA-B or DNA β, whereas, in the latter case, tomato leaf curl New Delhi virus DNA-B was found in association with the DNA-A of DLCV, like in many other Old World begomoviruses. The attempt to amplify DNA-B through PCR using newly designed primers NDB-F (5′-AAACACAGGAGGGATCGGAG-3′) and NDB-R (5′-CACAGATTTCCTTACGCGTATATT-3′) based on the sequence KT948071 was unsuccessful. Similarly, efforts to detect alphasatellite and betasatellite molecules in infected F. virens using universal primers (Bull et al. 2003; Briddon et al. 2002) were unsuccessful. Furthermore, Southern blot hybridization was carried out using [32P]-radiolabeled PCR product of 1 kb obtained with Beg-F/R primers as a probe. This produced dsDNA bands of different conformations as well as a ssDNA band, which confirms the presence of DLCV in F. virens. Members of the family Geminiviridae, to which begomoviruses belong, mainly infect herbaceous hosts, but they have also been reported in a few woody hosts, such as citrus chlorotic dwarf associated virus in citrus (Loconsole et al. 2012), grapevine red blotch virus in grape (Al Rwahnih et al. 2013), jatropha mosaic virus in Jatropha multifida (Polston et al. 2014), and apple geminivirus 1 in apple tree (Liang et al. 2015). To our knowledge, this is the first report of DLCV infecting F. virens in Pakistan. The results obtained here indicate that F. virens may serve as an alternative host of DLCV and a possible source of infection to the commonly grown D. erecta ornamental garden hedge.The author(s) declare no conflict of interest.References:Al Rwahnih, M., et al. 2013. Plant Dis. 103:1069. https://doi.org/10.1094/PHYTO-10-12-0253-R Google ScholarAnwar, S., et al. 2017. Arch. Virol. 163:809. https://doi.org/10.1007/s00705-017-3672-y Crossref, ISI, Google ScholarBriddon, R. 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Virol. 159:3131. https://doi.org/10.1007/s00705-014-2132-1 Crossref, ISI, Google ScholarThe author(s) declare no conflict of interest.Funding: Funding was provided by Animal and Plant Quarantine Agency (Z-1543086-2017-21-01).DetailsFiguresLiterature CitedRelated Vol. 104, No. 7 July 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionA cucumber plant with a light gray lesion of charcoal rot at the soil line and microsclerotia visible on the stem (D. S. Egel et al.). Photo credit: D. S. Egel. Rot and discolored sepals of persimmon fruit caused by Mucor inaequisporus (S.-Y. Lee and H.-Y. Jung). Photo credit: H.-Y. Jung. Metrics Article History Issue Date: 2 Jul 2020Published: 12 May 2020First Look: 12 Mar 2020Accepted: 11 Mar 2020 Page: 2034 Information© 2020 The American Phytopathological SocietyFundingAnimal and Plant Quarantine AgencyGrant/Award Number: Z-1543086-2017-21-01KeywordsFicus virensBegomovirusDuranta leaf curl virusThe author(s) declare no conflict of interest.Cited byFirst Report of Tomato Leaf Curl Bangladesh Virus Associated with Leaf Yellowing Symptoms in Lablab purpureus in BangladeshThuy T. B. Vo, Aamir Lal, Phuong T. Ho, Phuong T. Hoang, Kyeong-Yeoll Lee, Youngjae Chung, S. M. Hemayet Jahan, Eui-Joon Kil, and Sukchan Lee19 December 2022 | Plant Disease, Vol. 107, No. 2First Report of Squash leaf curl China virus Associated with Mosaic and Mild Leaf Curl Disease of Pumpkin in BangladeshThuy T. B. Vo, Eui-Joon Kil, Fadhila Chairina, Aamir Lal, Phuong T. Ho, Kyeong-Yeoll Lee, S. M. 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