Portal de Periódicos da CAPES

Oocyte activation, oolemma piercing, and real-time viability confirmation in human oocytes using electrophysiological techniques

2020; Lippincott Williams & Wilkins; Volume: 32; Issue: 3 Linguagem: Inglês

10.1097/gco.0000000000000625

1473-656X

Amir Mor, Emre Seli,

Pluripotent Stem Cells Research

To discuss the recent applications of electrophysiological principles to the optimization and automation of the IVF laboratory.There is growing evidence showing improvement of live birth rates following oocyte electro-activation. Novel applications using electrophysiological techniques are now employed to determine oocyte penetration and viability in real-time.In this short review, we summarize the recent advances in the integration of electrophysiological techniques into the assisted reproductive technology laboratories. We describe the potential clinical applications and their advantages such as creation of reliable automated cell injection systems and novel manual intracytoplasmic sperm injection (ICSI) training platforms. We also discuss theoretical adverse effects and ways to mitigate them.