Produção Nacional
Revisado por pares
Trypsin purified from Coryphaena hippurus (common dolphinfish): Purification, characterization, and application in commercial detergents
2020; Elsevier BV; Volume: 25; Linguagem: Inglês
10.1016/j.bcab.2020.101584
ISSN1878-8181
AutoresDávida Maria Ribeiro Cardoso dos Santos, Cláudio Wilian Victor dos Santos, Cledson Barros de Souza, Fabiana Sarmento de Albuquerque, José Marcos dos Santos Oliveira, Hugo Juarez Vieira Pereira,
Tópico(s)Aquaculture Nutrition and Growth
ResumoCoryphaena hippurus is a medium-to-large-sized fish very much appreciated in fine cooking. However, about 30% of its body, which corresponds to its viscera, is discarded and usually in an unlawful manner. When improperly disposed, this material causes environmental problems such as water and soil pollution. To minimize the issues caused by this organic waste, the use of fish viscera has increased as a source of important biomolecules like proteins, enzymes, and amino acids, which exhibit important characteristics that can be applied in biotechnology processes. Therefore, we aimed to purify and characterize trypsin from Coryphaena hippurus, and to study its application as an additive in commercial detergents. Trypsin was purified using fractionation by saline precipitation and collected at the 40%–60% fraction of ammonium sulfate, followed by molecular exclusion using a S-100 HR gel column. Trypsin from Coryphaena hippurus exhibits important characteristics, such as an optimum temperature of 40 °C and preservation of 50% of its enzymatic activity at 50 °C, an optimal pH of 8 and preservation of 70% of its enzymatic activity from pH 5 to 10. Km and Kcat were determined as 0.035 mM and 241.037 S−1, respectively, indicating high catalytic efficiency. Furthermore, enzymatic activity was still detected by zymography when added to all the commercial detergents tested. Our results suggest that trypsin purified from Coryphaena hippurus is a promising enzyme for application in biotechnology processes.
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