A High-Throughput Single-Clone Phage Fluorescence Microwell Immunoassay and Laser-Driven Clonal Retrieval System
2020; Multidisciplinary Digital Publishing Institute; Volume: 10; Issue: 4 Linguagem: Inglês
10.3390/biom10040517
ISSN2218-273X
AutoresSeohee Chang, Soohyun Kim, Jerome Han, Su-Ji Ha, Hyunho Lee, Seo Woo Song, Daewon Lee, Sunghoon Kwon, Junho Chung, Junhoi Kim,
Tópico(s)Nanofabrication and Lithography Techniques
ResumoPhage display is one of the most frequently used platform technologies utilized to screen and select therapeutic antibodies, and has contributed to the development of more than 10 therapeutic antibodies used in the clinic. Despite advantages like efficiency and low cost, it has intrinsic technical limitations, such as the asymmetrical amplification of the library after each round of biopanning, which is regarded as a reason for it yielding a very limited number of antigen binders. In this study, we developed a high-throughput single-clonal screening system comprised of fluorescence immunoassays and a laser-driven clonal DNA retrieval system using microchip technology. Using this system, from a single-chain variable fragment (scFv) library displayed on phages with a complexity of 5.21 × 10
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