First Report of Vascular Wilt on Lentil ( Lens culinaris ) Caused by Fusarium redolens in Pakistan
2020; American Phytopathological Society; Volume: 104; Issue: 9 Linguagem: Inglês
10.1094/pdis-11-19-2519-pdn
ISSN1943-7692
AutoresKhola Rafique, Seogchan Kang, Azizuddin, Tariq Mahmood, Imran Imran, Ihsan Ullah, H. Mahmood,
Tópico(s)Essential Oils and Antimicrobial Activity
ResumoHomePlant DiseaseVol. 104, No. 9First Report of Vascular Wilt on Lentil (Lens culinaris) Caused by Fusarium redolens in Pakistan PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Vascular Wilt on Lentil (Lens culinaris) Caused by Fusarium redolens in PakistanK. Rafique, S. Kang, Aziz-ud-Din, T. Mahmood, Imran, I. Ullah, and H. MahmoodK. Rafique†Corresponding author: K. Rafique; E-mail Address: kholarafique@gmail.comhttp://orcid.org/0000-0002-1646-3400Pest Warning and Quality Control of Pesticides, Department of Agriculture, Punjab, Lahore 54000, Pakistan, S. KangDepartment of Plant Pathology and Environmental Microbiology, The Pennsylvania State University, University Park, PA 16802, U.S.A., Aziz-ud-DinDepartment of Genetics, Hazara University, Mansehra 21120, Pakistan, T. MahmoodDepartment of Agriculture, Hazara University, Mansehra 21120, PakistanDepartment of Bioinformatics, Hazara University, Mansehra 21120, Pakistan, ImranDepartment of Agriculture, Hazara University, Mansehra 21120, Pakistan, I. UllahDepartment of Genetics, Hazara University, Mansehra 21120, Pakistan, and H. MahmoodDepartment of Bioinformatics, Hazara University, Mansehra 21120, Pakistan AffiliationsAuthors and Affiliations K. Rafique1 † S. Kang2 Aziz-ud-Din3 T. Mahmood4, 5 Imran4 I. Ullah3 H. Mahmood5 1Pest Warning and Quality Control of Pesticides, Department of Agriculture, Punjab, Lahore 54000, Pakistan 2Department of Plant Pathology and Environmental Microbiology, The Pennsylvania State University, University Park, PA 16802, U.S.A. 3Department of Genetics, Hazara University, Mansehra 21120, Pakistan 4Department of Agriculture, Hazara University, Mansehra 21120, Pakistan 5Department of Bioinformatics, Hazara University, Mansehra 21120, Pakistan Published Online:8 Jul 2020https://doi.org/10.1094/PDIS-11-19-2519-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Vascular wilt was observed in lentils (Lens culinaris Medikus) grown in four fields in the district of Narowal (32°06′N; 74°52′E) of Punjab, Pakistan, from 2011 to 2013. Symptoms included yellowing and stunting with vascular discoloration of the stem and root tissues. Thirty wilted plant samples were collected from each field. Small 8- to 10-mm infected root segments with visible symptoms were surface sterilized in 1% sodium hypochlorite for 2 min, rinsed thrice with sterile distilled water, plated on potato dextrose agar, and incubated at 25°C for 7 days. Isolates that exhibited morphological characteristics of Fusarium oxysporum Schlecht. emend. Snyder & Hansen (Leslie and Summerell 2006) were recovered from affected plants. On malt extract agar medium, colonies appeared white with appressed mycelium and light-brown pigmentation. Numerous macroconidia measuring 11.9 to 29 × 2.5 to 4 μm were produced. Macroconidia were three- to five-septate and straight, with a pointed apical cell and a prominent foot-shaped basal cell. Oval or cylindrical microconidia measuring 3.5 to 8.9 × 1.8 to 3.4 μm were abundant in false heads. Chlamydospores, measuring 7.2 to 17.8 μm, were spherical and were solitary or in short chains. Sequences of TEF-1α (Geiser et al. 2004) and ITS (White et al. 1990) from FWN2 isolate were submitted to GenBank as accession numbers KR011716 and MN537039, respectively. The two sequences shared 100% identity to Fusarium redolens with TEF-1α gene accession JF740748.1 and ITS accession KC924920. Although F. redolens and F. oxysporum are morphologically similar, they are phylogenetically distinct (O'Donnell et al. 1998). Based on these marker sequences, isolate FWN2 was identified as F. redolens Wollenw. The pathogenicity of FWN2 on lentil was evaluated twice. The roots of 15-day-old seedlings of cultivar Masoor-93 were inoculated by submerging them in a 1 × 107 conidia/ml suspension for 10 min, and they were potted in plastic pots (five seedlings per pot) containing sterilized potting mixture (sand/clay/farmyard manure, 1:1:1) and then maintained in a greenhouse at 25 ± 2°C. Seedlings dipped in sterile water served as the negative control. Inoculated seedlings developed wilt symptoms 15 days after inoculation and showed prominent discoloration of their stem and roots. After 20 days, 50% of the inoculated plants died. Noninoculated plants remained healthy. F. redolens was reisolated from wilted plants and confirmed based on morphological and molecular characteristics. This is the first report of F. redolens as a wilt pathogen on lentils in Pakistan. The presence of this new emerging pathogen in one of the main lentil-growing districts calls for broader surveys to assess whether this species poses a serious threat to lentil production in Pakistan.The author(s) declare no conflict of interest.References:Geiser, D. M., et al. 2004. Eur. J. Plant Pathol. 110:473. https://doi.org/10.1023/B:EJPP.0000032386.75915.a0 Crossref, ISI, Google ScholarLeslie, J. F., and Summerell, B. A. 2006. The Fusarium Laboratory Manual. Blackwell, Oxford, U.K. https://doi.org/10.1002/9780470278376 Crossref, Google ScholarO'Donnell, K., et al. 1998. Mycologia 90:465. https://doi.org/10.1080/00275514.1998.12026933 Crossref, ISI, Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA. Crossref, Google ScholarThe author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 104, No. 9 September 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionRipening strawberry infected by Colletotrichum acutatum (M. Dowling et al.). Photo credit: M. Dowling. Diaporthe leaf spot caused by Diaporthe humulicola on hop cone tissue (E. Allan-Perkins et al.). Photo credit: M. Salvas. Metrics Downloaded 674 times Article History Issue Date: 26 Aug 2020Published: 8 Jul 2020First Look: 6 Apr 2020Accepted: 1 Apr 2020 Pages: 2524-2524 Information© 2020 The American Phytopathological SocietyKeywordsfungifield cropsoilseeds and legumespathogen detectionThe author(s) declare no conflict of interest.Cited ByFirst report of Wilt on Lentil (Len culinaris Medik.) caused by Fusarium redolens in TunisiaSamira CHEKALI, Ali OUJI, Stefania SOMMA, Mario Masiello, Wala DOUIHECH, Eya Khemir, Antonio Moretti, and Samia Gargouri18 October 2021 | Plant Disease, Vol. 0, No. jaDuohua Huangjing (Polygonatum cyrtonema) Seedling Basal Stem Rot Caused by Fusarium redolens in ChinaTao Tang, Fanfan Wang, Jie Guo, Xiaoliang Guo, Yuanyuan Duan, and Jingmao You8 October 2021 | Plant Disease, Vol. 0, No. 0Molecular characterization and distribution of Fusarium isolates from uncultivated soils and chickpea plants in Iran with special reference to Fusarium redolens4 January 2021 | Journal of Plant Pathology, Vol. 103, No. 1Species-Specific Impact of Fusarium Infection on the Root and Shoot Characteristics of Asparagus24 June 2020 | Pathogens, Vol. 9, No. 6
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