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Freeform 3D printing using a continuous viscoelastic supporting matrix

2020; IOP Publishing; Volume: 12; Issue: 3 Linguagem: Inglês

10.1088/1758-5090/ab8bc3

1758-5090

Sónia G. Patrício, Liliana R. Sousa, Tiago R. Correia, Vítor M. Gaspar, Liliana Pires, Jorge Luís, José M. Oliveira, João F. Mano,

Additive Manufacturing and 3D Printing Technologies

Embedded bio-printing has fostered significant advances toward the fabrication of soft complex tissue-like constructs, by providing a physical support that allows the freeform shape maintenance within the prescribed spatial arrangement, even under gravity force. Current supporting materials still present major drawbacks for up-scaling embedded 3D bio-printing technology towards tissue-like constructs with clinically relevant dimensions. Herein, we report a a cost-effective and widely available supporting material for embedded bio-printing consisting on a continuous pseudo-plastic matrix of xanthan-gum (XG). This natural polisaccharide exhibits peculiar rheological properties that have enabled the rapid generation of complex volumetric 3D constructs with out-of-plane features. The freedom of design within the three orthogonal axes through the independent and controlled bio-printing process opens new opportunities to produce on demand large arbitrary shapes for personalized medicine. Additionally, we have demonstrated the versatile functionality of XG as a photocurable gel reservoir to engineer perfused cell-laden hydrogel constructs, addressing other practical biomedical applications such as in vitro models and organ-on-chip platforms.