The red fox ( Vulpes vulpes ) as a potential natural reservoir of human cryptosporidiosis by Cryptosporidium hominis in Northwest Spain
2020; Wiley; Linguagem: Inglês
10.1111/tbed.13569
ISSN1865-1682
AutoresJuan Pedro Barrera, David Carmena, Elena Rodríguez‐Rodríguez, Rocío Checa, Ana María López, Luis Eusebio Fidalgo, Rosa Gálvez, Valentina Marino, Isabel Fuentes, Guadalupe Miró, Ana Montoya,
Tópico(s)Parasitic Infections and Diagnostics
ResumoTransboundary and Emerging DiseasesVolume 67, Issue 5 p. 2172-2182 ORIGINAL ARTICLE The red fox (Vulpes vulpes) as a potential natural reservoir of human cryptosporidiosis by Cryptosporidium hominis in Northwest Spain Juan P. Barrera, Juan P. Barrera Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, SpainSearch for more papers by this authorDavid Carmena, Corresponding Author David Carmena dacarmena@isciii.es orcid.org/0000-0002-4015-8553 Laboratorio de Referencia e Investigación en Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain Correspondence David Carmena, Laboratorio de Referencia e Investigación en Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo Km 2, 28008 Majadahonda, Madrid, Spain. Email: dacarmena@isciii.es Guadalupe Miró, Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro s/n, 28040 Madrid. Spain. Email: gmiro@ucm.esSearch for more papers by this authorElena Rodríguez, Elena Rodríguez Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, SpainSearch for more papers by this authorRocío Checa, Rocío Checa Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, SpainSearch for more papers by this authorAna M. López, Ana M. López Departamento de Ciencias Clínicas Veterinarias, Facultad de Veterinaria, Universidad de Lugo, Lugo, SpainSearch for more papers by this authorLuis E. Fidalgo, Luis E. Fidalgo Departamento de Ciencias Clínicas Veterinarias, Facultad de Veterinaria, Universidad de Lugo, Lugo, SpainSearch for more papers by this authorRosa Gálvez, Rosa Gálvez Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, SpainSearch for more papers by this authorValentina Marino, Valentina Marino Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, SpainSearch for more papers by this authorIsabel Fuentes, Isabel Fuentes Laboratorio de Referencia e Investigación en Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, SpainSearch for more papers by this authorGuadalupe Miró, Guadalupe Miró Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, SpainSearch for more papers by this authorAna Montoya, Ana Montoya Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, SpainSearch for more papers by this author Juan P. Barrera, Juan P. Barrera Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, SpainSearch for more papers by this authorDavid Carmena, Corresponding Author David Carmena dacarmena@isciii.es orcid.org/0000-0002-4015-8553 Laboratorio de Referencia e Investigación en Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain Correspondence David Carmena, Laboratorio de Referencia e Investigación en Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo Km 2, 28008 Majadahonda, Madrid, Spain. Email: dacarmena@isciii.es Guadalupe Miró, Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro s/n, 28040 Madrid. Spain. Email: gmiro@ucm.esSearch for more papers by this authorElena Rodríguez, Elena Rodríguez Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, SpainSearch for more papers by this authorRocío Checa, Rocío Checa Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, SpainSearch for more papers by this authorAna M. López, Ana M. López Departamento de Ciencias Clínicas Veterinarias, Facultad de Veterinaria, Universidad de Lugo, Lugo, SpainSearch for more papers by this authorLuis E. Fidalgo, Luis E. Fidalgo Departamento de Ciencias Clínicas Veterinarias, Facultad de Veterinaria, Universidad de Lugo, Lugo, SpainSearch for more papers by this authorRosa Gálvez, Rosa Gálvez Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, SpainSearch for more papers by this authorValentina Marino, Valentina Marino Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, SpainSearch for more papers by this authorIsabel Fuentes, Isabel Fuentes Laboratorio de Referencia e Investigación en Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, SpainSearch for more papers by this authorGuadalupe Miró, Guadalupe Miró Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, SpainSearch for more papers by this authorAna Montoya, Ana Montoya Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, SpainSearch for more papers by this author First published: 17 April 2020 https://doi.org/10.1111/tbed.13569Citations: 8Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Giardia duodenalis and Cryptosporidium spp. are ubiquitous intestinal protozoa that parasitize domestic and wild animals, as well as human beings. Due to their zoonotic potential, the objective of the present study was to determine the presence of these pathogens in the fox population (Vulpes vulpes) located in Northwest Spain. A total of 197 faecal samples from legally hunted foxes were collected in the autonomous region of Galicia. The presence of G. duodenalis and Cryptosporidium spp. was investigated by PCR-based methods amplifying the small subunit ribosomal RNA (ssu rRNA) gene of the parasites. Attempts to genotype obtained positive samples were subsequently conducted at the glutamate dehydrogenase (gdh) and β-giardin (bg) genes of G. duodenalis, and the 60 kDa glycoprotein (gp60) gene of Cryptosporidium. Giardia duodenalis and Cryptosporidium spp. were identified in 19 (9.6%) and 12 (6.1%) of the investigated samples, respectively. However, five Cryptosporidium species were detected at the ssu rRNA locus: C. hominis (33.4%, 4/12), C. canis (25.0%, 3/12), C. parvum (16.7%, 2/12), C. ubiquitum (8.3%, 1/12) and C. suis (8.3%, 1/12). An additional Cryptosporidium-positive sample was identified at the genus level only. Typing and subtyping of Giardia- and Cryptosporidium-positive samples were unsuccessful. The detection of C. hominis in wild foxes indicates the probable overlapping of sylvatic and domestic cycles of this parasite in rural settings. Besides, this finding raises the question of whether red foxes may act as natural reservoirs of C. hominis. The detection of C. parvum and C. suis is suggestive of active transmission events between farm and wild animals, opening up the possibility of transmission to human beings. CONFLICT OF INTEREST The authors have no conflict of interest to declare. Open Research DATA AVAILABILITY STATEMENT The data that support the findings of this study are available in the supplementary material of this article. Citing Literature Volume67, Issue5September 2020Pages 2172-2182 RelatedInformation
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