Produção Nacional
Revisado por pares
First Report of Colletotrichum aeschynomenes and C. tropicale Causing Anthracnose on Myrciaria dubia in Brazil
2020; American Phytopathological Society; Volume: 104; Issue: 9 Linguagem: Inglês
10.1094/pdis-04-19-0882-pdn
ISSN1943-7692
AutoresKedma da Silva Matos, Joana Filipa Félix Machado, Pollyana Cardoso Chagas, R.H.S. Siqueira, G. F. Silva, Maria Santina Xavier Filha, Hyanameyka Evangelista de Lima-Primo, Edvan Alves Chagas,
Tópico(s)Banana Cultivation and Research
ResumoHomePlant DiseaseVol. 104, No. 9First Report of Colletotrichum aeschynomenes and C. tropicale Causing Anthracnose on Myrciaria dubia in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Colletotrichum aeschynomenes and C. tropicale Causing Anthracnose on Myrciaria dubia in BrazilK. S. Matos, J. F. Machado, P. C. Chagas, R. H. S. Siqueira, G. F. Silva, M. S. Xavier Filha, H. E. Lima-Primo, and E. A. ChagasK. S. Matos†Corresponding author: K. S. Matos; E-mail Address: kedma.ufla@gmail.comhttp://orcid.org/0000-0003-4118-6449Departamento de Fitotecnia, Universidade Federal de Roraima, Campus Cauamé, 69300-000, Boa Vista, Roraima, BrazilSearch for more papers by this author, J. F. MachadoDepartamento de Fitotecnia, Universidade Federal de Roraima, Campus Cauamé, 69300-000, Boa Vista, Roraima, BrazilSearch for more papers by this author, P. C. ChagasDepartamento de Fitotecnia, Universidade Federal de Roraima, Campus Cauamé, 69300-000, Boa Vista, Roraima, BrazilSearch for more papers by this author, R. H. S. SiqueiraInstituto Federal de Roraima, 69343-000, Amajari, Roraima, BrazilSearch for more papers by this author, G. F. SilvaEmbrapa Amazônia Ocidental, 69010-970, Manaus, Amazonas, BrazilSearch for more papers by this author, M. S. Xavier FilhaEmbrapa Roraima, 69301-970, Boa Vista, Roraima, BrazilSearch for more papers by this author, H. E. Lima-PrimoEmbrapa Roraima, 69301-970, Boa Vista, Roraima, BrazilSearch for more papers by this author, and E. A. ChagasEmbrapa Roraima, 69301-970, Boa Vista, Roraima, BrazilSearch for more papers by this authorAffiliationsAuthors and Affiliations K. S. Matos1 † J. F. Machado1 P. C. Chagas1 R. H. S. Siqueira2 G. F. Silva3 M. S. Xavier Filha4 H. E. Lima-Primo4 E. A. Chagas4 1Departamento de Fitotecnia, Universidade Federal de Roraima, Campus Cauamé, 69300-000, Boa Vista, Roraima, Brazil 2Instituto Federal de Roraima, 69343-000, Amajari, Roraima, Brazil 3Embrapa Amazônia Ocidental, 69010-970, Manaus, Amazonas, Brazil 4Embrapa Roraima, 69301-970, Boa Vista, Roraima, Brazil Published Online:22 Jul 2020https://doi.org/10.1094/PDIS-04-19-0882-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Camu-camu (Myrciaria dubia [Kunth] McVaugh) is a native fruit tree of Amazon region that produces fruit with high content of vitamin C. The enormous economic potential has encouraged its domestication process in the northern region of Brazil. Since 2016, brown, necrotic, anthracnose-like lesions were observed on 30 to 40% of the camu-camu plants in an experimental field located in the city of Boa Vista, Roraima, Brazil (2°52′20.7″ N; 60°42′44.2″ W). Small pieces of tissue taken from the lesions were surface disinfested previously (1 min in 70% alcohol, 1 min in 1% NaOCl, and rinsed with sterile water). Leaf sections were plated on water agar medium, incubated at 25°C for 4 days, and afterward were transferred to potato dextrose agar (PDA) medium. After 7 days on PDA at 25°C, the isolate CA3B formed a grayish-white colony. Conidia were hyaline, aseptate, subcylindrical with rounded ends, measuring (n = 20) 12.1 to 15.9 × 4.9 to 7.0 μm. Appressoria were brown, subglobose, clavate, measuring 7.4 to 16.7 × 4.6 to 9.9 μm. The isolate COL02 formed light gray colonies. Conidia were hyaline, aseptate, cylindrical, tapering slightly near both ends, measuring (n = 20) 12.3 to 16.1 × 4.7 to 6.5 μm. Appressoria were brown, mostly elliptic to subfusoid, deeply lobed, measuring 8.7 to 15.9 × 5.8 to 9.9 μm. Single-spore cultures were deposited in the Microorganisms Culture Collection of EMBRAPA Roraima, Roraima, Brazil. Total DNA was extracted, and fragments of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), β-tubulin 2 (TUB2), glutamine synthetase (GS), and manganese-superoxide dismutase (SOD2) genes were amplified (Weir et al. 2012) and sequenced. The obtained sequences showed 94 to 100% identity with sequences published in GenBank of the Colletotrichum gloeosporioides species complex, with a predominance of C. tropicale, C. aeschynomenes, and C. siamense. Sequences from the two isolates were deposited in GenBank (accession nos.: GAPDH, MK792453, MK792457; GS, MK792454, MK792458; SOD2, MK792455, MK792459; and TUB2, MK792456, MK792460). Phylogenetic Bayesian inference analysis based in a combined data set (concatenated sequences of GAPDH, TUB2, GS, and SOD2) showed that the isolate CA3B clustered with the ex-holotype specimen of C. tropicale (CBS 124949), and the isolate COL02 clustered with the ex-type specimen of C. aeschynomenes (ICMP 17673) with high support (posterior probability = 1). To confirm the pathogenicity, five seedlings of the camu-camu were sprayed with 106 conidia/ml spore suspension for each isolate. Five seedlings were sprayed with sterile water as a negative control. All seedlings were covered with plastic bags 24 h after inoculation and kept at 27°C in a greenhouse with a 12-h photoperiod. After 7 days, depressed and necrotic lesions were observed in the inoculated leaves. In the third treatment, both isolates were coinoculated. Negative control plants showed no symptoms. To fulfill Koch’s postulates, both species were successfully reisolated from the inoculated and coinoculated leaves by cultural morphology. In Brazil, C. tropicale causes anthracnose on different hosts, such as mango and soursop (Costa et al. 2019; Lima et al. 2013). C. aeschynomenes was associated with Aeschynomene virginica in the United States (Weir et al. 2012), cassava in Thailand (Sangpueak et al. 2018), and cacao in Brazil (Nascimento et al. 2019). To our knowledge, this is the first report of C. aeschynomenes and C. tropicale causing anthracnose on M. dubia worldwide.The author(s) declare no conflict of interest.References:Costa, J. F. O., et al. 2019. Eur. J. Plant Pathol. 153:1119. https://doi.org/10.1007/s10658-018-01630-w Crossref, ISI, Google ScholarLima, N. B., et al. 2013. Fungal Divers. 61:75. https://doi.org/10.1007/s13225-013-0237-6 Crossref, ISI, Google ScholarNascimento, D. A., et al. 2019. Plant Dis. 103:3284. https://doi.org/10.1094/PDIS-11-18-2047-PDN Link, Google ScholarSangpueak, R., et al. 2018. J. Phytopathol. 166:129. https://doi.org/10.1111/jph.12669 Crossref, ISI, Google ScholarWeir, B. S., et al. 2012. Stud. Mycol. 73:115. https://doi.org/10.3114/sim0011 Crossref, ISI, Google ScholarThe author(s) declare no conflict of interest.Funding: Funding was provided by Empresa Brasileira de Pesquisa Agropecuária.DetailsFiguresLiterature CitedRelated Vol. 104, No. 9 September 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionRipening strawberry infected by Colletotrichum acutatum (M. Dowling et al.). Photo credit: M. Dowling. Diaporthe leaf spot caused by Diaporthe humulicola on hop cone tissue (E. Allan-Perkins et al.). Photo credit: M. Salvas. Metrics Article History Issue Date: 26 Aug 2020Published: 22 Jul 2020First Look: 17 Apr 2020Accepted: 15 Apr 2020 Pages: 2517-2517 Information© 2020 The American Phytopathological SocietyFundingEmpresa Brasileira de Pesquisa AgropecuáriaKeywordsColletotrichumnative fruit treecamu camuThe author(s) declare no conflict of interest.Cited byFirst Report of Anthracnose of Sanhua Plum Caused by Colletotrichum aeschynomenes in Guangxi, ChinaM. M. Lu, L. A. Ma, L. H. Tang, X. L. Chen, T. X. Guo, J. Y. Mo, S. P. Huang, and Q. L. Li7 March 2023 | Plant Disease, Vol. 0, No. 0First Report of Colletotrichum tropicale Causing Leaf Spot on Cariniana legalis in BrazilT. M. Souza, J. Pereira, J. L. Bezerra, and R. J. V. de Oliveira29 January 2023 | Plant Disease, Vol. 107, No. 2Fungal diversity notes 1512–1610: taxonomic and phylogenetic contributions on genera and species of fungal taxa23 February 2023 | Fungal Diversity, Vol. 117, No. 1Colletotrichum tropicaleCABI Compendium, Vol. CABI CompendiumColletotrichum species and complexes: geographic distribution, host range and conservation status29 September 2021 | Fungal Diversity, Vol. 110, No. 1
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