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Inhibiting Monocyte Recruitment to Prevent the Pro-Tumoral Activity of Tumor-Associated Macrophages in Chondrosarcoma

2020; Multidisciplinary Digital Publishing Institute; Volume: 9; Issue: 4 Linguagem: Inglês

10.3390/cells9041062

2073-4409

Michele Minopoli, Sabrina Sarno, Gioconda Di Carluccio, Rosa Azzaro, Susan Costantini, Flavio Fazioli, Michele Gallo, Gaetano Apice, L. Cannella, Domenica Rea, Maria Patrizia Stoppelli, Diana Boraschi, Alfredo Budillon, Katia Scotlandi, Annarosaria De Chiara, Maria Vincenza Carriero,

Sarcoma Diagnosis and Treatment

Chondrosarcomas (CHS) are malignant cartilaginous neoplasms with diverse morphological features, characterized by resistance to chemo- and radiation therapies. In this study, we investigated the role of tumor-associated macrophages (TAM)s in tumor tissues from CHS patients by immunohistochemistry. Three-dimensional organotypic co-cultures were set up in order to evaluate the contribution of primary human CHS cells in driving an M2-like phenotype in monocyte-derived primary macrophages, and the capability of macrophages to promote growth and/or invasiveness of CHS cells. Finally, with an in vivo model of primary CHS cells engrafted in nude mice, we tested the ability of a potent peptide inhibitor of cell migration (Ac-d-Tyr-d-Arg-Aib-d-Arg-NH