First Report of Cotton Leafroll Dwarf Virus in Florida
2020; American Phytopathological Society; Volume: 104; Issue: 10 Linguagem: Inglês
10.1094/pdis-10-19-2150-pdn
ISSN1943-7692
AutoresFanny Iriarte, K. K. Dey, Ian M. Small, Kassie Conner, G. Kelly O’Brien, Ladd E. Johnson, C. Savery, E. Carter, Danielle Sprague, R. L. Nichols, David L. Wright, M. J. Mulvaney, Mathews L. Paret,
Tópico(s)Research in Cotton Cultivation
ResumoHomePlant DiseaseVol. 104, No. 10First Report of Cotton Leafroll Dwarf Virus in Florida Previous DISEASE NOTES OPENOpen Access licenseFirst Report of Cotton Leafroll Dwarf Virus in FloridaF. B. Iriarte, K. K. Dey, I. M. Small, K. N. Conner, G. K. O'Brien, L. Johnson, C. Savery, E. Carter, D. Sprague, R. L. Nichols, D. L. Wright, M. J. Mulvaney, and M. ParetF. B. IriarteUniversity of Florida, IFAS, North Florida Research & Education Center, Quincy, FL, K. K. DeyFlorida Department of Agriculture and Consumer Services Division of Plant Industry, Gainesville, FL, I. M. Small†Corresponding author: I. M. Small; E-mail Address: ismall@ufl.eduhttp://orcid.org/0000-0001-5406-3486University of Florida, IFAS, North Florida Research & Education Center, Quincy, FL, K. N. ConnerAlabama Cooperative Extension System, Auburn University, Auburn AL, G. K. O'BrienUniversity of Florida, IFAS, North Florida Research & Education Center, Quincy, FL, L. JohnsonUniversity of Florida, IFAS Escambia County Extension, Cantonment, FL, C. SaveryAnchor Ag Solutions, Montrose, AL, E. CarterUniversity of Florida, IFAS Jackson County Extension, Marianna, FL, D. SpragueUniversity of Florida, IFAS Jefferson County Extension, Monticello, FL, R. L. NicholsCotton Inc., Cary, NC, D. L. WrightUniversity of Florida, IFAS, North Florida Research & Education Center, Quincy, FL, M. J. MulvaneyUniversity of Florida, IFAS, West Florida Research & Education Center, Jay, FL, and M. Parethttp://orcid.org/0000-0002-2520-0418University of Florida, IFAS, North Florida Research & Education Center, Quincy, FLAffiliationsAuthors and Affiliations F. B. Iriarte1 K. K. Dey2 I. M. Small1 † K. N. Conner3 G. K. O'Brien1 L. Johnson4 C. Savery5 E. Carter6 D. Sprague7 R. L. Nichols8 D. L. Wright1 M. J. Mulvaney9 M. Paret1 1University of Florida, IFAS, North Florida Research & Education Center, Quincy, FL 2Florida Department of Agriculture and Consumer Services Division of Plant Industry, Gainesville, FL 3Alabama Cooperative Extension System, Auburn University, Auburn AL 4University of Florida, IFAS Escambia County Extension, Cantonment, FL 5Anchor Ag Solutions, Montrose, AL 6University of Florida, IFAS Jackson County Extension, Marianna, FL 7University of Florida, IFAS Jefferson County Extension, Monticello, FL 8Cotton Inc., Cary, NC 9University of Florida, IFAS, West Florida Research & Education Center, Jay, FL Published Online:27 Jul 2020https://doi.org/10.1094/PDIS-10-19-2150-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Cotton leafroll dwarf disease, caused by the Cotton leafroll dwarf virus (CLRDV), is an emerging disease of cotton (Gossypium hirsutum L.) in the United States. It was previously reported in Alabama (Avelar et al. 2019), Mississippi (Aboughanem-Sabanadzovic et al. 2019), Georgia (Tabassum et al. 2019), Kansas (Ali and Mokhtari 2020), North Carolina (Huseth 2019), and Texas (Alabi et al. 2020). To date, three CLRDV genotypes have been reported throughout the world: "typical", "atypical", and North American CLRDV-AL (Avelar et al. 2019). During the 2019 growing season in several counties in north Florida, cotton seedlings and mature plants exhibited stunting, leaf distortion, yellowing, and/or reddening. A total of 232 symptomatic and asymptomatic plant samples from 12 Florida counties were tested for the presence of CLRDV. Total RNA was purified from lower stems, petioles, and/or leaves using the Zymo Quick-RNA Microprep kit (Genesee Scientific Corporation, U.S.A.). Complementary DNA (cDNA) was obtained using GoScript Reverse Transcription Mix (Promega, U.S.A.). Polymerase chain reaction (PCR) was performed using CLRDV primers AL674F/AL1407R (Avelar et al. 2019), to amplify the partial P1 (ORF1) and P0 (ORF0) region of the genome. CLRDV cDNA provided by K. N. Conner at Auburn University was used as the positive control. No cDNA was used as the negative control. An amplicon of the expected size, 733 bp, was obtained from all 102 symptomatic samples but not from 130 asymptomatic samples. Purified reverse transcription PCR products of 19 representative Florida isolates from the above-mentioned counties were subjected to Sanger sequencing. NCBI BLASTn analysis of the resulting sequences revealed 100% identity to CLRDV isolate from Alabama (MH883237) (Avelar et al. 2019), 94% identity to "typical" isolates from Argentina (GU167940) and Brazil (HQ827780), and 92% identity to "atypical" isolates from Argentina (KF359946) and Brazil (KF906261). Phylogenetic analysis using MEGA X (Kumar et al. 2018) was also conducted to support previous results. Three genotypes were observed. Three representative sequences from this analysis were submitted to GenBank: Escambia County (Co.) isolates ES312A (MT036373) and ES229 (MT036374), and Santa Rosa Co. isolate SR225 (MT036375). For 21 CLRDV isolates, an additional set of primers, CLRDV3675F/Pol3982R (Sharman et al. 2015), was used to amplify the partial ORF3 of the virus encoding the coat protein. An amplicon of the expected size, 310 bp, was obtained from all these isolates. PCR products were purified and directly sequenced. NCBI BLASTn analysis of resulting sequences revealed 100% identity to the CLRDV Alabama isolate (MH883236) and 99% identity to a Brazilian "typical" isolate (HQ827780). Phylogenetic analysis using MEGA X (Kumar et al. 2018) of these 21 CLRDV isolates resulted in two genotypes. Three representative sequences from two genotypes were submitted to GenBank: Gadsden Co. isolate GA219 (MT036376), Jefferson Co. isolate JE223 (MT036377), and Santa Rosa Co. isolate SR225s (MT036378). CLRDV was detected in 12 counties in Florida with high prevalence in Gadsden, Jefferson, and Santa Rosa counties. More research is needed on the transmission, epidemiology, virulence, and host tolerance to this virus, which will help establish best management practices.The author(s) declare no conflict of interest.References:Aboughanem-Sabanadzovic, N., et al. 2019. Plant Dis. 103:1798. https://doi.org/10.1094/PDIS-01-19-0017-PDN Link, ISI, Google ScholarAlabi, O. J., et al. 2020. Plant Dis. 104:998. https://doi.org/10.1094/PDIS-09-19-2008-PDN Google ScholarAli, A. and Mokhtari, S. 2020. doi: 10.1094/PDIS-12-19-2589-PDN. doi.org/10.1094/PDIS-12-19-2589-PDN Google ScholarAvelar, S., et al. 2019. Plant Dis. 103:592. https://doi.org/10.1094/PDIS-09-18-1550-PDN Google ScholarHuseth, A. 2019. Detection of cotton leafroll dwarf virus in North Carolina. https://www.cottonfarming.com/production-2/detection-of-cotton-leafroll-dwarf-virus-in-north-carolina/ Google ScholarKumar, S., et al. 2018. Mol. Biol. Evol. 35:1547. https://doi.org/10.1093/molbev/msy096 Crossref, ISI, Google ScholarSharman, M., et al. 2015. Australas. Plant Dis. Notes 10:24. https://doi.org/10.1007/s13314-015-0174-1 Crossref, ISI, Google ScholarTabassum, A., et al. 2019. Plant Dis. 103:1803. https://doi.org/10.1094/PDIS-12-18-2197-PDN Link, ISI, Google ScholarThe author(s) declare no conflict of interest.Funding: Funding was provided by Cotton Incorporated.DetailsFiguresLiterature CitedRelated Vol. 104, No. 10 October 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionSymptoms of yellow leaf disease of Areca catechu caused by areca palm velarivirus 1 (H. X. Wang et al.). Photo credit: X. Huang. Fungal fruiting bodies of Phyllachora maydis on corn foliage resemble spots of tar (J. Valle-Torres et al.). Photo credit: C. Cruz. Geranium (Pelargonium hortorum) showing pale green and little leaves, phyllody, virescence, and witches'-broom (A. R. Amirmijani et al.). Photo credit: M. Azadvar. Metrics Article History Issue Date: 25 Sep 2020Published: 27 Jul 2020First Look: 21 Apr 2020Accepted: 17 Apr 2020 Page: 2744 Information© 2020 The American Phytopathological SocietyFundingCotton IncorporatedKeywordsviruses and viroidsfield cropspathogen detectioncottonCLRDVThe author(s) declare no conflict of interest.Cited byProspective Alternate Hosts of an Emerging Polerovirus in Cotton Landscapes in the Southeastern United States13 October 2022 | Viruses, Vol. 14, No. 10Investigating the effects of planting date and Aphis gossypii management on reducing the final incidence of cotton leafroll dwarf virusCrop Protection, Vol. 158Complete Genome Sequence of Cotton Leafroll Dwarf Virus Infecting Cotton in Oklahoma, USAMicrobiology Resource Announcements, Vol. 11, No. 7Antibodies for the Coat Protein of Cotton Leafroll Dwarf Virus Detect Commelina sp. as an Intermediary Host for Cotton Blue Disease13 July 2022 | Frontiers in Plant Science, Vol. 13Cotton Leafroll Dwarf Virus US Genomes Comprise Divergent Subpopulations and Harbor Extensive Variability5 November 2021 | Viruses, Vol. 13, No. 11Effect of Cotton Leafroll Dwarf Virus on Physiological Processes and Yield of Individual Cotton Plants1 October 2021 | Frontiers in Plant Science, Vol. 12Genome analysis of cotton leafroll dwarf virus reveals variability in the silencing suppressor protein, genotypes and genomic recombinants in the USA7 July 2021 | PLOS ONE, Vol. 16, No. 7Natural host range, incidence on overwintering cotton and diversity of cotton leafroll dwarf virus in Georgia USACrop Protection, Vol. 144Genome Sequence of Cotton Leafroll Dwarf Virus Infecting Cotton in Georgia, USAMicrobiology Resource Announcements, Vol. 9, No. 34
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