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Different Signatures of High Cardiorespiratory Capacity Revealed With Metabolomic Profiling in Elite Athletes

2020; Human Kinetics; Volume: 15; Issue: 8 Linguagem: Inglês

10.1123/ijspp.2019-0267

1555-0273

Gustavo Monnerat, Carlos Almonacid Sanchez, Caleb Guedes Miranda dos Santos, Dailson Paulucio, Rodolfo Velasque, Geisa Paulino Caprini Evaristo, Joseph Albert Medeiros Evaristo, Fábio César Sousa Nogueira, Gilberto B. Domont, Mauricio Serrato, Antonio S. Lima, David J. Bishop, Antônio Carlos Campos de Carvalho, Fernando Pompeu,

Adipose Tissue and Metabolism

High cardiorespiratory capacity is a key determinant of human performance and life expectancy; however, the underlying mechanisms are not fully understood. The objective of this pilot study was to investigate biochemical signatures of endurance-performance athletes using high-resolution nontargeted metabolomics.Elite long-distance runners with similar training and anthropometrical records were studied. After athletes' maximal oxygen consumption (V˙O2max) was measured, they were divided into 2 groups: low V˙O2max ( 75 mL·kg-1·min-1, n = 7). Plasma was collected under basal conditions after 12 hours of fasting and after a maximal exercise test (nonfasted) and analyzed by high-resolution LC-MS. Multivariate and univariate statistics were applied.A total of 167 compounds were putatively identified with an LC-MS-based metabolomics pipeline. Partial least-squares discriminant analysis showed a clear separation between groups. Significant variations in metabolites highlighted group differences in diverse metabolic pathways, including lipids, vitamins, amino acids, purine, histidine, xenobiotics, and others, either under basal condition or after the maximal exercise test.Taken together, the metabolic alterations revealed in the study affect cellular energy use and availability, oxidative stress management, muscle damage, central nervous system signaling metabolites, nutrients, and compound bioavailability, providing new insights into metabolic alterations associated with exercise and cardiorespiratory fitness levels in trained athletes.