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BR-Bodies Provide Selectively Permeable Condensates that Stimulate mRNA Decay and Prevent Release of Decay Intermediates

2020; Elsevier BV; Volume: 78; Issue: 4 Linguagem: Inglês

10.1016/j.molcel.2020.04.001

1097-4164

Nadra Al-Husini, Dylan T. Tomares, Zechariah Pfaffenberger, Nisansala S. Muthunayake, Mohammad Abdus Samad, Tiancheng Zuo, Obaidah Bitar, James R. Aretakis, Mohammed-Husain M. Bharmal, Alisa Gega, Julie S. Biteen, W. Seth Childers, Jared M. Schrader,

RNA modifications and cancer

Biomolecular condensates play a key role in organizing RNAs and proteins into membraneless organelles. Bacterial RNP-bodies (BR-bodies) are a biomolecular condensate containing the RNA degradosome mRNA decay machinery, but the biochemical function of such organization remains poorly defined. Here, we define the RNA substrates of BR-bodies through enrichment of the bodies followed by RNA sequencing (RNA-seq). We find that long, poorly translated mRNAs, small RNAs, and antisense RNAs are the main substrates, while rRNA, tRNA, and other conserved non-coding RNAs (ncRNAs) are excluded from these bodies. BR-bodies stimulate the mRNA decay rate of enriched mRNAs, helping to reshape the cellular mRNA pool. We also observe that BR-body formation promotes complete mRNA decay, avoiding the buildup of toxic endo-cleaved mRNA decay intermediates. The combined selective permeability of BR-bodies for both enzymes and substrates together with the stimulation of the sub-steps of mRNA decay provide an effective organization strategy for bacterial mRNA decay.