First Report of Pomegranate Fruit Rot Caused by Botryosphaeria dothidea in Anhui Province of China
2020; American Phytopathological Society; Volume: 104; Issue: 10 Linguagem: Inglês
10.1094/pdis-04-20-0790-pdn
ISSN1943-7692
AutoresChunyan Gu, Yang Xue, Mohamed N. Al-Attala, Muhammad Abid, Seinn Sandar May Phyo, Hao-Yu Zang, Rui Pan, Yu Chen,
Tópico(s)Fungal Plant Pathogen Control
ResumoHomePlant DiseaseVol. 104, No. 10First Report of Pomegranate Fruit Rot Caused by Botryosphaeria dothidea in Anhui Province of China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Pomegranate Fruit Rot Caused by Botryosphaeria dothidea in Anhui Province of ChinaChun-Yan Gu, Xue Yang, Mohamed N. Al-Attala, Muhammad Abid, Seinn Sandar May Phyo, Hao-Yu Zang, Rui Pan, and Yu ChenChun-Yan GuInstitute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, ChinaScientific Observing and Experimental Station of Crop Pests in Hefei, Ministry of Agriculture, ChinaLaboratory of Quality and Safety Risk Assessment for Agro-Products, Hefei, Ministry of Agriculture, ChinaAnhui Province Key Laboratory of Monitoring and Management of Pest Resistance, Anhui Academy of Agricultural Sciences, Hefei 230031, China, Xue YangInstitute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, ChinaScientific Observing and Experimental Station of Crop Pests in Hefei, Ministry of Agriculture, ChinaLaboratory of Quality and Safety Risk Assessment for Agro-Products, Hefei, Ministry of Agriculture, ChinaAnhui Province Key Laboratory of Monitoring and Management of Pest Resistance, Anhui Academy of Agricultural Sciences, Hefei 230031, China, Mohamed N. Al-Attalahttp://orcid.org/0000-0001-6916-8328Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, ChinaScientific Observing and Experimental Station of Crop Pests in Hefei, Ministry of Agriculture, ChinaLaboratory of Quality and Safety Risk Assessment for Agro-Products, Hefei, Ministry of Agriculture, ChinaAnhui Province Key Laboratory of Monitoring and Management of Pest Resistance, Anhui Academy of Agricultural Sciences, Hefei 230031, ChinaPlant Pathology Unit, Plant Protection Department, Desert Research Center, Cairo 11753, Egypt, Muhammad Abidhttp://orcid.org/0000-0003-3870-0830Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, ChinaScientific Observing and Experimental Station of Crop Pests in Hefei, Ministry of Agriculture, ChinaLaboratory of Quality and Safety Risk Assessment for Agro-Products, Hefei, Ministry of Agriculture, ChinaAnhui Province Key Laboratory of Monitoring and Management of Pest Resistance, Anhui Academy of Agricultural Sciences, Hefei 230031, ChinaDepartment of Plant Pathology, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan 60800, Pakistan, Seinn Sandar May PhyoInstitute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, ChinaScientific Observing and Experimental Station of Crop Pests in Hefei, Ministry of Agriculture, ChinaLaboratory of Quality and Safety Risk Assessment for Agro-Products, Hefei, Ministry of Agriculture, ChinaAnhui Province Key Laboratory of Monitoring and Management of Pest Resistance, Anhui Academy of Agricultural Sciences, Hefei 230031, ChinaMolecular Genetic Laboratory, Biotechnology Research Department, Kyaukse 05151, Mandalay Region, Myanmar, Hao-Yu ZangInstitute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, ChinaScientific Observing and Experimental Station of Crop Pests in Hefei, Ministry of Agriculture, ChinaLaboratory of Quality and Safety Risk Assessment for Agro-Products, Hefei, Ministry of Agriculture, ChinaAnhui Province Key Laboratory of Monitoring and Management of Pest Resistance, Anhui Academy of Agricultural Sciences, Hefei 230031, China, Rui PanInstitute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, ChinaScientific Observing and Experimental Station of Crop Pests in Hefei, Ministry of Agriculture, ChinaLaboratory of Quality and Safety Risk Assessment for Agro-Products, Hefei, Ministry of Agriculture, ChinaAnhui Province Key Laboratory of Monitoring and Management of Pest Resistance, Anhui Academy of Agricultural Sciences, Hefei 230031, China, and Yu Chen†Corresponding author: Y. Chen; E-mail Address: chenyu66891@sina.comhttp://orcid.org/0000-0001-9767-5255Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, ChinaScientific Observing and Experimental Station of Crop Pests in Hefei, Ministry of Agriculture, ChinaLaboratory of Quality and Safety Risk Assessment for Agro-Products, Hefei, Ministry of Agriculture, ChinaAnhui Province Key Laboratory of Monitoring and Management of Pest Resistance, Anhui Academy of Agricultural Sciences, Hefei 230031, China AffiliationsAuthors and Affiliations Chun-Yan Gu1 2 3 4 Xue Yang1 2 3 4 Mohamed N. Al-Attala1 2 3 4 5 Muhammad Abid1 2 3 4 6 Seinn Sandar May Phyo1 2 3 4 7 Hao-Yu Zang1 2 3 4 Rui Pan1 2 3 4 Yu Chen1 2 3 4 † 1Institute of Plant Protection and Agro-products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, China 2Scientific Observing and Experimental Station of Crop Pests in Hefei, Ministry of Agriculture, China 3Laboratory of Quality and Safety Risk Assessment for Agro-Products, Hefei, Ministry of Agriculture, China 4Anhui Province Key Laboratory of Monitoring and Management of Pest Resistance, Anhui Academy of Agricultural Sciences, Hefei 230031, China 5Plant Pathology Unit, Plant Protection Department, Desert Research Center, Cairo 11753, Egypt 6Department of Plant Pathology, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan 60800, Pakistan 7Molecular Genetic Laboratory, Biotechnology Research Department, Kyaukse 05151, Mandalay Region, Myanmar Published Online:4 Aug 2020https://doi.org/10.1094/PDIS-04-20-0790-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Pomegranate (Punica granatum L.) is an important commercial fruit rich in antioxidants grown worldwide, and China has the biggest planting area in the world. In spring 2019, symptoms of fruit rot were observed for the first time in some orchards located in Huaiyuan County of Anhui Province, one of the main pomegranate-producing areas in China. Disease symptoms on fruit were characterized by enlarging and merging coarse irregular spots that coalesced and became large decay lesions on the fruit surface. Infected fruits developed internal rot, became discolored, and dropped. Aggregated pycnidia were found in a black stroma on fallen mummified fruit. For isolation of the pathogen, more than 20 infected pomegranate fruit samples were collected from an orchard. After surface cleaning with sterile water to clear away some dust and other microbes, drying, disinfecting with 70% ethanol for 1 min, and rinsing twice with sterile water, small pieces with diseased and healthy tissue parts were placed on water agar plates (WA). A small piece of every fungal isolate grown on the WA was transferred onto potato dextrose agar (PDA) and incubated at 28°C for 5 days. All fungal colonies on PDA plates gradually changed from gray to dark gray with an olive green back and abundant aerial mycelia, bearing pycnidia with fusiform, hyaline, and 16.2 to 20.1 × 3.8 to 5.4 μm (n = 50) in size. Twenty isolates with similar cultural characteristics were stored for further work. The internal transcribed spacer (ITS) region, β-tubulin gene (TUB), and translation elongation factor 1-α (EF 1-α) gene were amplified and sequenced from three typical isolates using the universal primer pairs ITS1/ITS4 (White et al. 1990), Bt2a/Bt2b (Glass and Donaldson 1995), and EF1-728F/EF1-986R (Carbone and Kohn 1999), respectively. The BLAST results of ITS (MN453421), TUB (MN515421), and EF 1-α (MN515422) sequences showed 99.64, 99.07, and 98.89% identity with the Botryosphaeria dothidea sequences available in the GenBank database (KP708996, MH724212, and KP183206), respectively. To complete Koch's postulates, healthy fresh pomegranate fruits were disinfected with 70% ethanol solution for 1 min and rinsed with distilled water. Fruits were allowed to dry and inoculated on the wounded surface using a mycelial plug 5 mm in diameter excised from the edges of actively growing fungal colonies; fruits inoculated with PDA plugs served as a negative control. Three fruits were used for each treatment and control. These fruits were kept under high humidity (95% relative humidity) using sterile distilled water in an artificial incubator, and the experiment was repeated twice. Decayed lesions resembling natural infections were observed on fruits after 5 to 7 days of inoculation. Reisolation of the pathogen, which was also identified based on the above morphological and molecular analysis as B. dothidea, produced the same fungus as the one used for inoculating the fruit, thus completing Koch's postulates. Therefore, the pathogen of this pomegranate fruit rot in China is B. dothidea. Botryosphaeria pomegranate rot caused by B. dothidea in Florida has been reported previously, and it was hypothesized that this fungus could be transmitted by leaf-footed bug (Leptoglossus clypealis, Michailides 2014). To our knowledge, this is the first report of B. dothidea causing fruit rot on pomegranate in China.The author(s) declare no conflict of interest.References:Carbone, I., and Kohn, L. M. 1999. Mycologia 91:553. https://doi.org/10.1080/00275514.1999.12061051 Crossref, ISI, Google ScholarGlass, N. L., and Donaldson, G. C. 1995. Appl. Environ. Microbiol. 61:1323. https://doi.org/10.1128/AEM.61.4.1323-1330.1995 Crossref, ISI, Google ScholarMichailides, T. J. 2014. Botrysphaeria: A Manageable Disease in Pomegranates? Florida Pomegranate Association 3rd Annual Meeting. Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA. Crossref, Google ScholarThe author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 104, No. 10 October 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionSymptoms of yellow leaf disease of Areca catechu caused by areca palm velarivirus 1 (H. X. Wang et al.). Photo credit: X. Huang. Fungal fruiting bodies of Phyllachora maydis on corn foliage resemble spots of tar (J. Valle-Torres et al.). Photo credit: C. Cruz. Geranium (Pelargonium hortorum) showing pale green and little leaves, phyllody, virescence, and witches'-broom (A. R. Amirmijani et al.). Photo credit: M. Azadvar. Metrics Downloaded 416 times Article History Issue Date: 25 Sep 2020Published: 4 Aug 2020First Look: 24 Apr 2020Accepted: 21 Apr 2020 Pages: 2736-2736 Information© 2020 The American Phytopathological SocietyKeywordspomegranate diseasepomegranate fruit rotBotryosphaeria dothideaITSEF 1-ALPHABETA-tubulin geneThe author(s) declare no conflict of interest.Cited ByEndophytic Bacterium Serratia plymuthica From Chinese Leek Suppressed Apple Ring Rot on Postharvest Apple Fruit3 March 2022 | Frontiers in Microbiology, Vol. 12Efficacy of Dimethyl Trisulfide on the Suppression of Ring Rot Disease Caused by Botryosphaeria dothidea and Induction of Defense-Related Genes on Apple Fruits7 February 2022 | Frontiers in Microbiology, Vol. 13
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