Immunological Methods
1970; Elsevier BV; Linguagem: Inglês
10.1016/b978-0-08-015685-9.50005-2
AutoresH. Van Cauwenberge, P Franchimont, A Luyckx,
Tópico(s)Monoclonal and Polyclonal Antibodies Research
ResumoThis chapter discusses the immunological methods. The introduction of immunological techniques into the field of endocrinology constituted a considerable advance. With these techniques, it became possible to assay in vivo a series of protein hormones that are intimately involved in regulating the body's metabolic processes. The injection of a protein hormone obtained from one species of animal readily induces the formation of antibody in an animal of another species, provided the hormone has a molecular weight of more than 10,000. The chemical structure of these hormones presents a species-specificity that is very helpful in the production of antibodies; the precise degree of species-specificity varies with the hormone considered. The chapter also discusses the techniques for visualizing the immunochemical reaction. These techniques may be perturbed by nonspecific factors, in which case they may be responsible for a lack of specificity. Adsorption of the hormone to wood charcoal was proposed for insulin and later for other hormones by Herbert.
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