First Report of Cotton Leafroll Dwarf Virus from Upland Cotton ( Gossypium hirsutum ) in Arkansas
2020; American Phytopathological Society; Volume: 104; Issue: 10 Linguagem: Inglês
10.1094/pdis-12-19-2610-pdn
ISSN1943-7692
AutoresTravis Faske, Daisy Stainton, Nina Aboughanem‐Sabanadzovic, Thomas Wesley Allen,
Tópico(s)Plant Disease Resistance and Genetics
ResumoHomePlant DiseaseVol. 104, No. 10First Report of Cotton Leafroll Dwarf Virus from Upland Cotton (Gossypium hirsutum) in Arkansas PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Cotton Leafroll Dwarf Virus from Upland Cotton (Gossypium hirsutum) in ArkansasTravis R. Faske, Daisy Stainton, Nina Aboughanem-Sabanadzovic, and Tom W. AllenTravis R. Faske†Corresponding author: T. R. Faske; E-mail Address: tfaske@uaex.eduhttp://orcid.org/0000-0002-5441-1775Lonoke Extension Center, University of Arkansas Division of Agriculture, Lonoke, AR 72086, Daisy StaintonDepartment of Entomology and Plant Pathology, University of Arkansas Division of Agriculture, Fayetteville, AR 72701, Nina Aboughanem-SabanadzovicInstitute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi State, MS 39762, and Tom W. Allenhttp://orcid.org/0000-0003-2828-3420Delta Research and Extension Center, Mississippi State University, Stoneville, MS 38776AffiliationsAuthors and Affiliations Travis R. Faske1 † Daisy Stainton2 Nina Aboughanem-Sabanadzovic3 Tom W. Allen4 1Lonoke Extension Center, University of Arkansas Division of Agriculture, Lonoke, AR 72086 2Department of Entomology and Plant Pathology, University of Arkansas Division of Agriculture, Fayetteville, AR 72701 3Institute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi State, MS 39762 4Delta Research and Extension Center, Mississippi State University, Stoneville, MS 38776 Published Online:18 Aug 2020https://doi.org/10.1094/PDIS-12-19-2610-PDNAboutSections ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Cotton leafroll dwarf virus (CLRDV; genus Polerovirus, family Luteoviridae) is a positive-sense single-stranded RNA virus with a 5.8-kb genome that has been associated with cotton blue disease (Corrêa et al. 2005; Distéfano et al. 2010). Recently, CLRDV was reported in several cotton-producing states in the United States, including Alabama, Georgia, Kansas, Mississippi, Oklahoma, South Carolina, and Texas (Aboughanem-Sabanadzovic et al. 2019; Alabi et al. 2020; Ali and Mokhtari 2020; Ali et al. 2020; Avelar et al. 2019, 2020; Tabassum et al. 2019; Wang et al. 2020). Following these reports, an investigation of CLRDV was initiated in Arkansas. During the fall of 2019 (September to November) a survey was conducted in six cotton-producing counties (Chicot, Desha, Drew, Lee, Monroe, and Phillips) in eastern Arkansas. A range of symptoms previously associated with CLRDV-infected cotton were observed, which included shortened internodes in the terminal part of the canopy, leaf distortion, upward leaf cupping, reddish-brown aborted flower bracts, and reduced boll set in the eight fields sampled. Overall, a disease incidence of <1% was visually observed in individual fields. Field samples consisted of the terminal growth (top 15 cm) from at least six plants that expressed CLRDV-like symptoms from each field. A total of 26 samples were tested in two separate reverse transcription PCR (RT-PCR) experiments targeting the viral polymerase and coat protein (CP) genes. RT-PCR experiments were performed on total RNA, extracted from combined petiole and leaf material using the Qiagen RNeasy kit following manufacturer's protocol, using primers CLRDV-RdRpF2/CLRDV-RdRpR1 and CLRDV-CPF1/CLRDV-CPR1, which amplified a region of the RdRp (770 bp) and CP (249 bp) genes, respectively (Aboughanem-Sabanadzovic et al. 2019). Six of the 26 samples tested produced DNA bands of the expected size in both PCR reactions, indicating the presence of CLRDV in Chicot, Desha, and Lee counties. To confirm that RT-PCR results were CLRDV-specific, PCR products from two CLRDV-positive samples collected in Chicot and Lee counties were Sanger sequenced. Comparison of CLRDV partial sequences of the two isolates from Arkansas (MN787063 and MT332312 for RdRp; MN787064 and MT332313 for CP) shared the highest percentage identity (99.4%) for the RdRp region with a Mississippi isolate (MK512764) and 100% identity for the CP region to one isolate from Alabama (MN071395), two from Georgia (MK290759 to 60), and five from Mississippi (MK512765 to 66, MK512768 to 70). Partial genome sequence comparison of RdRp and CP regions of the two CLRDV isolates from Arkansas revealed that they were virtually identical. Currently, CLRDV is of major concern within the cotton industry, and this report confirms that the virus is widely distributed in the United States. Additional research is needed to better understand the impact of CLRDV in Arkansas and in the U.S. cotton production system.The author(s) declare no conflict of interest.References:Aboughanem-Sabanadzovic, N., et al. 2019. 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Plant Dis. 103:1803. https://doi.org/10.1094/PDIS-12-18-2197-PDN Link, ISI, Google ScholarWang, H., et al. 2020. Plant Dis. https://doi.org/10.1094/PDIS-03-20-0635-PDN Google ScholarThe author(s) declare no conflict of interest.Funding: This research funded in part through USDA-ARS agreements 58-6066-6-046 and 58-6066-6-059, USDA-ARS NACA agreement 58-6066-9-033, and Cotton Inc. grant 19-207.DetailsFiguresLiterature CitedRelated Vol. 104, No. 10 October 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionSymptoms of yellow leaf disease of Areca catechu caused by areca palm velarivirus 1 (H. X. Wang et al.). Photo credit: X. Huang. Fungal fruiting bodies of Phyllachora maydis on corn foliage resemble spots of tar (J. Valle-Torres et al.). Photo credit: C. Cruz. Geranium (Pelargonium hortorum) showing pale green and little leaves, phyllody, virescence, and witches'-broom (A. R. Amirmijani et al.). Photo credit: M. Azadvar. Metrics Article History Issue Date: 25 Sep 2020Published: 18 Aug 2020First Look: 7 May 2020Accepted: 5 May 2020 Page: 2742 Information© 2020 The American Phytopathological SocietyFundingUSDA-ARSGrant/Award Number: 58-6066-6-046Grant/Award Number: 58-6066-6-059USDA-ARS NACAGrant/Award Number: 58-6066-9-033Cotton Inc.Grant/Award Number: 19-207Keywordscottonvirus diagnosiscotton leafroll dwarf virusThe author(s) declare no conflict of interest.Cited byProspective Alternate Hosts of an Emerging Polerovirus in Cotton Landscapes in the Southeastern United States13 October 2022 | Viruses, Vol. 14, No. 10Complete Genome Sequence of Cotton Leafroll Dwarf Virus Infecting Cotton in Oklahoma, USAMicrobiology Resource Announcements, Vol. 11, No. 7Cotton Leafroll Dwarf Virus US Genomes Comprise Divergent Subpopulations and Harbor Extensive Variability5 November 2021 | Viruses, Vol. 13, No. 11Effect of Cotton Leafroll Dwarf Virus on Physiological Processes and Yield of Individual Cotton Plants1 October 2021 | Frontiers in Plant Science, Vol. 12Genome analysis of cotton leafroll dwarf virus reveals variability in the silencing suppressor protein, genotypes and genomic recombinants in the USA7 July 2021 | PLOS ONE, Vol. 16, No. 7Natural host range, incidence on overwintering cotton and diversity of cotton leafroll dwarf virus in Georgia USACrop Protection, Vol. 144
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