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Transcutaneous Administration of Dengue Vaccines

2020; Multidisciplinary Digital Publishing Institute; Volume: 12; Issue: 5 Linguagem: Inglês

10.3390/v12050514

1999-4915

Robert Andreata‐Santos, Rúbens Prince dos Santos Alves, Sara Araújo Pereira, Lennon Ramos Pereira, Carla Longo de Freitas, Samuel Santos Pereira, Aléxia Adrianne Venceslau-Carvalho, Maria Fernanda de Castro‐Amarante, Marianna Teixeira de Pinho Favaro, Camila Mathias-Santos, Jaime Henrique Amorim, Luís Carlos de Souza Ferreira,

Viral Infections and Outbreaks Research

In the present study, we evaluated the immunological responses induced by dengue vaccines under experimental conditions after delivery via a transcutaneous (TC) route. Vaccines against type 2 Dengue virus particles (DENV2 New Guinea C (NGC) strain) combined with enterotoxigenic Escherichia coli (ETEC) heat-labile toxin (LT) were administered to BALB/c mice in a three-dose immunization regimen via the TC route. As a control for the parenteral administration route, other mouse groups were immunized with the same vaccine formulation via the intradermic (ID) route. Our results showed that mice vaccinated either via the TC or ID routes developed similar protective immunity, as measured after lethal challenges with the DENV2 NGC strain. Notably, the vaccine delivered through the TC route induced lower serum antibody (IgG) responses with regard to ID-immunized mice, particularly after the third dose. The protective immunity elicited in TC-immunized mice was attributed to different antigen-specific antibody properties, such as epitope specificity and IgG subclass responses, and cellular immune responses, as determined by cytokine secretion profiles. Altogether, the results of the present study demonstrate the immunogenicity and protective properties of a dengue vaccine delivered through the TC route and offer perspectives for future clinical applications.