Produção Nacional
Histological and Ultrastructural Analysis of Tilapia rendalli Liver as an Environmental Assessment Tool for Cachoeira River, Bahia, Brazil
2019; Volume: 06; Issue: 01 Linguagem: Inglês
10.33745/ijzi.2020.v06i01.003
ISSN2454-3055
AutoresFábio Flores-Lopes, Milane Alves Correia, Daniela Mariano Lopes da Silva,
Tópico(s)Aquatic Ecosystems and Phytoplankton Dynamics
ResumoDue to the degradation of aquatic ecosystems, environmental monitoring studies have been developed with the aid of conventional and biological tools.The objective of this study was to evaluate the environmental quality of the Cachoeira River, Bahia, Brazil by physico-chemical analysis of water and histopathological and ultrastructural analysis of Tilapia rendalli (Boulenger, 1897) liver.Samples were collected at six points in the municipalities of Itapé, Itabuna and Ilhéus, between 2010 and 2012.Water samples were filtered through 0.7 µm GF/F glass-microfiber membranes, and ion analysis (Mg +2 , Ca 2+ , K + , Na + , Cl -, NH4 + , NO3 -, PO4 3-, SO4 2-) was performed by ion-exchange chromatography.Fish specimens were collected using a seine-type drag net and fixed in 10% formalin.For histological analysis, the samples were embedded in paraffin, sectioned and stained with hematoxylineosin.For ultrastructural analysis, the samples were embedded in Epon resin, and sections were prepared and contrasted with 0.5% uranyl acetate.Semi-quantitative histopathological analysis were performed based on the severity of alterations.There were high concentrations of nutrients in water samples, and at all points sampled, the specimens showed histopathological and ultrastructural changes.Points 1, 2, 3 and 4 were characterized as moderate to severe both for histopathological and ultrastructural changes, indicating that these points had greater degradation than the others.
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