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Production and Purification of Zika Virus NS1 Glycoprotein in HEK293 Cells

2020; Springer Science+Business Media; Linguagem: Inglês

10.1007/978-1-0716-0581-3_8

1940-6029

Young Chan Kim, Nallely García‐Larragoiti, César López‐Camacho, Martha Eva Viveros‐Sandoval, Arturo Reyes‐Sandoval,

Virology and Viral Diseases

In this chapter, we describe production and purification of the Zika virus NS1 glycoprotein in human embryonic kidney (HEK293T) cells at small, research laboratory scale. The expression of secreted NS1 (sNS1) and the C-terminal β-ladder domain in HEK293T cells were tested in a small-scale transfection before scaling up to a larger-scale transfection using roller bottles. Two different purification approaches have been applied to obtain purified NS1 (sNS1) and the C-terminal β-ladder domain ready for clinical applications.