Cytotoxic Lymphocytes
1979; Elsevier BV; Linguagem: Inglês
10.1016/b978-0-12-635505-5.50009-3
AutoresPeter Perlmann, Jean‐Charles Cerottini,
Tópico(s)Immunotoxicology and immune responses
ResumoThis chapter discusses cytotoxic lymphocytes. Evaluation of the biological significance of the results of an in vitro assay is critically dependent on the correct assessment of the many limitations of the assay. Lymphocytes are mixed with proper target cells, and the ensuing damage of the latter is measured after incubation under tissue culture conditions. Thus, the outcome of the assay will depend on a large number of variables involving effector cells, target cells, and the assay itself. Moreover, cytotoxicity of a certain lymphocyte preparation can usually be expressed only in relative terms, that is, in comparison to a control constituting the baseline for the assay. While the choice of controls is crucial, the decision as to what constitutes the proper control may be very difficult. Most early assays relied on visual assessment of target cell death or growth inhibition after incubation with lymphocytes. In some procedures, functional inactivation of the target cells by the lymphocytes was measured in a similar way.
Referência(s)