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Astaxanthin alleviates oxidative damage in acute pancreatitis via direct antioxidant mechanisms

2020; Volume: 31; Issue: 10 Linguagem: Inglês

10.5152/tjg.2020.19520

2148-5607

Dilek Özbeyli, Esra Bihter Gürler, Hülya Buzcu, Özlem Kaya, Muhammet Emin Çam, Meral Yüksel,

Liver Disease Diagnosis and Treatment

Background/Aims: Astaxanthin (ATX) is a naturally occurring carotenoid and a potent antioxidant.Various anti-inflammatory effects of ATX have been examined.We aimed to investigate the protective effect of ATX and its mechanism in a cerulein-induced acute pancreatitis rat model. Materials and Methods:The rats were randomized into 2 main groups as control (C) and acute pancreatitis group (AP).AP group was subsequently divided into subgroups as AP+vehicle (AP), AP+ATX, and ATX+peroxisome proliferator-activated receptor-alpha antagonist GW6471 (ATX+GW) groups.To induce AP, the rats were administered cerulein (50 µg/kg, intraperitonally [ip]) at 1 hour intervals, whereas the C group received saline.The AP group was treated with vehicle olive oil, ATX 40 mg/kg/orally, or GW6471 and ATX (GW1 mg/kg/ip; ATX; 40 mg/kg/peroral).Treatments were administered after the 1st cerulein injection.At the 7 th hour after the final injection, the rats were killed and the pancreatic tissue was used for the determination of malondialdehyde (MDA), glutathione (GSH), and myeloperoxidase (MPO) activities and luminol-lucigenin chemiluminescence levels.Serum amylase, lipase, and histopathological analyses were performed.Results: Elevated serum lipase and amylase levels in the vehicle-treated AP group (p<0.01)decreased in the ATX and ATX+GW groups (p<0.05).In the AP groups, GSH was reduced and MDA, MPO, luminol, and lucigenin levels were increased (p<0.05-0.001).ATX reversed these changes (p<0.05-0.001).The vehicle-treated group revealed significant severe cytoplasmic degeneration and vacuolization, whereas ATX ameliorated these destructions.GW6471 did not abolish the positive effects of ATX biochemically or histologically.Conclusion: ATX has a potent protective effect on AP via its radical scavenging and antioxidant properties.Therefore, we believe that ATX may have therapeutic potential.