LXR Activation Down-regulates Lipid Raft Markers FLOT2 and DHHC5 in MCF-7 Breast Cancer Cells
2017; International Institute of Anticancer Research (IIAR) Conferences 1997. Athens, Greece. Abstracts; Volume: 37; Issue: 8 Linguagem: Inglês
10.21873/anticanres.11792
ISSN1791-7530
AutoresDelphine Carbonnelle, Trang H. Luu, Chloé Chaillou, Jean‐Michel Huvelin, Jean‐Marie Bard, Hassan Nazih,
Tópico(s)Cholesterol and Lipid Metabolism
ResumoLipid rafts are cholesterol-enriched microdomains of the plasma membrane. Recent studies have underlined that their integrity is critical for cancer cell survival. Liver X receptor (LXR) has a central role in cellular cholesterol homeostasis and its stimulation inhibits proliferation of several cancer cell lines. This study investigated whether LXR could modulate lipid rafts integrity and consequently alter proliferation of the MCF-7 breast cancer cell line.Effect of LXR agonist T0901317 on integrity of MCF-7 lipid rafts was examined by studying the expression of rafts marker flotillin-2 (FLOT2) and DHHC5, which palmitoylates FLOT2, and by studying the expression of phospho-Akt.We demonstrated that LXR stimulation decreases mRNA and protein expression of FLOT2 and DHHC5 in MCF-7 cells. LXR stimulation also reduces Akt phosphorylation and its localization at the plasma membrane.We showed, for the first time, that LXR regulates transcription of specific proteins of lipid rafts in a breast cancer model.
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