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Artigo Acesso aberto Produção Nacional
BIBTEX RIS

A comparative study of extraction techniques for maximum recovery of bioactive compounds from Ganoderma lucidum spores

2020; Volume: 49; Issue: 1 Linguagem: Inglês

10.15446/rcciquifa.v49n1.84456

ISSN

1909-6356

Autores

Diana Carolina Saavedra Plazas, Carlos Ricardo Soccol, Miguel D. Noseda, Valcineide Oliveira de Andrade Tanobe, Oranys Marín, Susan Grace Karp, Gilberto Vinícius de Melo Pereira, Júlio César de Carvalho, Vanete Thomaz‐Soccol,

Tópico(s)

Algal biology and biofuel production

Resumo

This study aimed at evaluating effective methods for breaking the hard and insoluble spores of Ganoderma lucidum to recover functional biomolecules. Rupture techniques were evaluated such as manual maceration (RM), maceration with spheres of various materials (BR), and microwave exposure plus maceration with steel/chrome spheres (MBR1). Spore rupture was evaluated using UV-Vis spectroscopy, which showed vibrations of 2955, 1642, 1240, 1080 and 1746 cm-1 corresponding to changes in spore walls. The MBR1 extract contained the largest amounts of carbohydrates (19.80 mg.g-1 spores) and polyphenols (2.21 mg.g-1 spores), whereas the BR extract had higher antioxidant activity (57.22%Inb DPPH). The MBR1 and BR extracts contained 62.2 and 73.5% glucose, respectively. Both methods also involved significant extraction of carbohydrates and proteins. The best way to extract biomolecules from spore walls is to perform a microwave heat treatment and break the walls with steel/chrome spheres; this produces large quantities of carbohydrates with antioxidant properties.

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