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Hybrid nanosilicas produced by the Stöber sol-gel process: In vitro evaluation in MRC-5 cells

2020; Elsevier BV; Volume: 542; Linguagem: Inglês

10.1016/j.jnoncrysol.2020.120152

1873-4812

Jussânia Gnoatto, João Vitor de Oliveira, Eduarda Arndt, Franciele Faccio Busatto, Yolice Patricia Moreno Ruiz, Ana Cristina Borba da Cunha, Dinara Jaqueline Moura, João Henrique Zimnoch dos Santos,

Polymer Surface Interaction Studies

• Series of 12 hybrid SiNPexhibiting different steric and electronic properties. • The nature of the organosilane alters particle size and colloidal stability. • SiNP-PEG, ZWT or C1moieties improved the physic-chemical properties of the SiNP. • SiNP- PEG and ZWT increased the cell biocompatibility when compared to bare SiNP. • Functionalized and nonfunctionalizedSiNP did not cause any damage to cellular DNA. A series of 12 organosilanes exhibiting different steric and electronic properties were employed in the synthesis of hybrid silicas by the Stöber sol-gel process. The resulting materials were characterized by a set of complementary techniques (nitrogen adsorption, dynamic light scattering, small-angle X-ray scattering, transmission electron microscopy, and carbon content analysis).. Hybrid silicas bearing polyethylene glycol (PEG), zwitterion (ZWT), and methyl (C1) moieties improved the physical-chemical properties of the nanoparticles for biological applications. Toxicological safety tests were performed on the fibroblast cell line (MRC-5), which showed that the functionalization of silica nanoparticles with PEG and ZWT increased cell viability, 111 ± 8.5% and 114 ± 6.3%, respectively, when compared to that of nanomaterials without functionalization, 98 ± 8.8%. Both functionalized and non-functionalized silica nanoparticles did not cause any damage to the DNA of the cells, even at the highest doses.