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Transglycosylic Reactions of Nucleotides of Deoxy‐Sugars

1973; Wiley; Volume: 40; Issue: 1 Linguagem: Inglês

10.1111/j.1432-1033.1973.tb03185.x

1432-1033

Jiří Zemek, Š. Kučár, Štefan Bauer,

Microbial Metabolic Engineering and Bioproduction

Incorporation of 3‐deoxy‐, 4‐deoxy‐, and 6‐deoxy‐analogues of d ‐glucose into glycogen was investigated in a reaction catalyzed with yeast UDPglucogen glucosyl transferase. It was found that hydroxyl groups at positions C‐3, C‐4 and C‐6 in the glucosyl moiety of the UDPG molecule are not essential for preserving its substrate properties in this transglycosylic reaction. The kinetics of 3‐deoxy‐, 4‐deoxy‐ and 6‐deoxy‐glucose incorporation into glycogen was compared with that of d ‐glucose and 2‐deoxy‐glucose. It was found that the affinity of the enzyme and the rate of hexose incorporation into glycogen decreases in following sequence: d ‐glucose, 2‐deoxy‐, 6‐deoxy‐, 4‐deoxy‐ and 3‐deoxyglucose. The deoxyglucosyl analogues of UDPG act as competitive inhibitors on the reaction of glycogen biosynthesis.