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Artigo Acesso aberto Produção Nacional Revisado por pares
BIBTEX RIS

Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19

2020; Elsevier BV; Volume: 282; Linguagem: Inglês

10.1016/j.jviromet.2020.113888

ISSN

1879-0984

Autores

Gúbio Soares Campos, Sílvia Inês Sardi, Melissa Barreto Falcão, Emilia M.M.A. Belitardo, Danilo J. P. G. Rocha, Carolina de Araújo Rolo, Aline Dórea Luz Menezes, Carina S. Pinheiro, Rejane Hughes Carvalho, João Paulo P. de Almeida, Eric Roberto Guimarães Rocha Aguiar, Luis G. C. Pacheco,

Tópico(s)

Bacteriophages and microbial interactions

Resumo

Herein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use of an optimized in-house developed bioinformatics strategy contributed to successful detection of the virus.

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