Cloning and expression in Escherichia coli of tryptophan genes from Streptomyces griseus IMRU 3570
1990; Oxford University Press; Volume: 68; Issue: 1-2 Linguagem: Inglês
10.1111/j.1574-6968.1990.tb04149.x
ISSN1574-6968
AutoresOctavio Miguel Rivero-Lezcano, Juan Anguita-Castillo, J. López-Nieto, Gérmán Naharro-Carrasco,
Tópico(s)Fungal and yeast genetics research
ResumoThe Sau3A fragments of Streptomyces grisues IMRU 3750 were cloned in pBR322 as a vector. One of these clones contained the genetic information needed to complement trpA and trpB mutations in Escherichia coli. The other complements trpA, trpB and trpC mutations in E. coli. Both fragments originated in the same region of the chromosome but the latter is 1 kilobase (kb) longer in the region nearest the tetracycline promoter.
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