Artigo Acesso aberto Produção Nacional Revisado por pares

Effect of low intensity photobiomodulation associated with norbixin-based poly (hydroxybutyrate) membrane on post-tenotomy tendon repair. In vivo study

2020; Sociedade Brasileira Para o Desenvolvimento da Pesquisa em Cirurgia; Volume: 35; Issue: 3 Linguagem: Inglês

10.1590/s0102-865020200030000003

ISSN

1678-2674

Autores

Lízia Daniela e Silva Nascimento, Renata Amadei Nicolau, Antônio Luíz Martins Maia Filho, Kárita Francisca e Silva Nascimento, José Zilton Lima Verde Santos, Rayssilane Cardoso de Sousa, Luiz Fernando Meneses Carvalho, Vicente Galber Freitas Viana,

Tópico(s)

Bee Products Chemical Analysis

Resumo

To evaluate the in vivo response of photobiomodulation therapy associated with norbixin-based poly(hydroxybutyrate) membrane (PHB) in tenotomized calcaneal tendon.Thirty rats were randomly allocated to six groups (n=5 each): LED groups (L1, L2 and L3) and membrane + LED groups (ML1, ML2 and ML3). The right calcaneal tendons of all animals were sectioned transversely and were irradiated with LED daily, one hour after surgery every 24 hours, until the day of euthanasia. At the end of the experiments the tendons were removed for histological analysis.The histological analysis showed a significant reduction in inflammatory cells in the ML1, ML2 and ML3 groups (p=0.0056, p=0.0018 and p<0.0001, respectively) compared to those in the LED group. There was greater proliferation of fibroblasts in the ML1 (p<0.0001) and L3 (p<0.0001) groups. A higher concentration of type I collagen was also observed in the ML1 group (p=0.0043) replacing type III collagen.Photobiomodulation in association with norbixin-based PHB membrane led to control of the inflammatory process. However, it did not favor fibroblast proliferation and did not optimize type I collagen formation in the expected stage of the repair process.

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