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Anti-Inflammatory and Physicochemical Characterization of the Croton rhamnifolioides Essential Oil Inclusion Complex in β-Cyclodextrin

2020; Multidisciplinary Digital Publishing Institute; Volume: 9; Issue: 6 Linguagem: Inglês

10.3390/biology9060114

2079-7737

Anita Oliveira Brito Pereira Bezerra Martins, Almir Gonçalves Wanderley, Isabel Sousa Alcântara, Lindaiane Bezerra Rodrigues, Francisco Rafael Alves Santana Cesário, Maria Rayane Correia de Oliveira, Fyama Ferreira e Castro, Thaís Rodrigues de Albuquerque, Maria Sanádia Alexandre da Silva, Jaime Ribeiro‐Filho, Henrique Douglas Melo Coutinho, Paula dos Passos Menezes, Lucindo José Quintans‐Júnior, Adriano Antunes de Souza Araújo, Marcello Iriti, Jackson Roberto Guedes da Silva Almeida, Irwin Rose Alencar de Menezes,

Phytochemistry and Bioactive Compounds

Croton rhamnifolioides is used in popular medicine for the treatment of inflammatory diseases. The objective of this study was to characterize and evaluate the anti-inflammatory effect of C. rhamnifolioides essential oil complexed in β-cyclodextrin (COEFC). The physicochemical characterization of the complexes was performed using different physical methods. The anti-inflammatory activity was evaluated in vivo by ear edema, paw edema, cotton pellet-induced granuloma, and vascular permeability by Evans blue extravasation. The mechanism of action was validated by molecular docking of the major constituent into the cyclooxygenase-2 (COX-2 enzyme). All doses of the COEFC reduced acute paw edema induced by carrageenan and dextran, as well as vascular permeability. Our results suggest the lowest effective dose of all samples inhibited the response induced by histamine or arachidonic acid as well as the granuloma formation. The complexation process showed that the pharmacological effects were maintained, however, showing similar results using much lower doses. The results demonstrated an involvement of the inhibition of pathways dependent on eicosanoids and histamine. Complexation of β-cyclodextrin/Essential oil (β-CD/EO) may present an important tool in the study of new compounds for the development of anti-inflammatory drugs.