First Report of Arthrocladiella mougeotii Causing Powdery Mildew on Lycium chinense in Henan, China
2020; American Phytopathological Society; Volume: 104; Issue: 11 Linguagem: Inglês
10.1094/pdis-02-20-0445-pdn
ISSN1943-7692
AutoresMo Zhu, Miao Zhao, Jie Ji, Chenhui Yang, Jinyan Chai, Yong-Fang Li,
Tópico(s)Yeasts and Rust Fungi Studies
ResumoHomePlant DiseaseVol. 104, No. 11First Report of Arthrocladiella mougeotii Causing Powdery Mildew on Lycium chinense in Henan, China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Arthrocladiella mougeotii Causing Powdery Mildew on Lycium chinense in Henan, ChinaMo Zhu, Miao Zhao, Jie Ji, Chenhui Yang, Jinyan Chai, and Yong-Fang LiMo Zhu†Corresponding authors: M. Zhu; E-mail Address: zhumo@htu.edu.cn and Y.-F. Li; E-mail Address: li_yongfang@hotmail.comhttp://orcid.org/0000-0003-0884-1368College of Life Sciences, Henan Normal University, Xinxiang 453007, P.R. China, Miao ZhaoCollege of Life Sciences, Henan Normal University, Xinxiang 453007, P.R. China, Jie JiCollege of Life Sciences, Henan Normal University, Xinxiang 453007, P.R. China, Chenhui YangCollege of Life Sciences, Henan Normal University, Xinxiang 453007, P.R. China, Jinyan ChaiCollege of Life Sciences, Henan Normal University, Xinxiang 453007, P.R. China, and Yong-Fang Li†Corresponding authors: M. Zhu; E-mail Address: zhumo@htu.edu.cn and Y.-F. Li; E-mail Address: li_yongfang@hotmail.comCollege of Life Sciences, Henan Normal University, Xinxiang 453007, P.R. ChinaAffiliationsAuthors and Affiliations Mo Zhu † Miao Zhao Jie Ji Chenhui Yang Jinyan Chai Yong-Fang Li † College of Life Sciences, Henan Normal University, Xinxiang 453007, P.R. China Published Online:16 Sep 2020https://doi.org/10.1094/PDIS-02-20-0445-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleChinese matrimony-vine (Lycium chinense Mill.) is a widely distributed perennial landscape plant in China. It is a precious germplasm resource for cultivation, and its fruit is used as a traditional Chinese medicine (Yossa Nzeuwa et al. 2019). During October and November 2019, powdery mildew was observed on leaves of L. chinense in several locations (e.g., the campus of He'nan Normal University, Renmin Park, and Muye Park) of Xinxiang City, Henan Province, China. White powdery colonies were observed on adaxial and abaxial surfaces of leaves. Infected leaves were collected, and the morphological characteristics of the pathogen were determined microscopically. Three to four foot cells were about 35 μm long, and conidiophores (n = 25) were 70 to 120 × 6 to 10 μm (length × width). Conidia were cylindrical or elliptical and were 18 to 28 × 5 to 18 μm, average (n = 50) 23 × 9 μm, with a length/width ratio of 2.6. No teleomorph was observed. Based on these morphologic characteristics, the pathogen was initially identified as Arthrocladiella mougeotii (Braun and Cook 2012). Infected leaf surfaces were painted with cellulose acetate/acetone solution (5%, w/v), fungal mycelia were stripped after evaporation of acetone, and genomic DNA of the pathogen was extracted using the method described previously (Zhu et al. 2019). The rDNA internal transcribed spacer (ITS) region was amplified with fungus universal primer pair PMITS1/PMITS2 (Cunnington et al. 2003). The PCR product was sequenced by Invitrogen (Shanghai, China), and the resulting sequence of 725 bp was deposited in GenBank as accession number MT007282. BLASTn analysis revealed that MT007282 was 100% identical to A. mougeotii at 633 of 633 nt (accession no. AB022380). Pathogenicity was tested by carefully dusting conidia from infested leaves onto the upper surfaces of young leaves from three healthy plants. As a control, three noninoculated plants were incubated. Inoculated and control plants were then maintained separately in growth chambers (light/dark: 16 h/8 h; temperature: 18 to 20°C). White powdery mildew symptoms were visible on inoculated plants leaves 7 to 12 days postinoculation and were morphologically identical to those observed on the original diseased leaves, whereas control plants remained powdery mildew-free. Therefore, the causal organism of the powdery mildew was A. mougeotii, confirmed by morphological characteristics and ITS sequence data. A. mougeotii has been reported on L. chinense in the United States and some locations (e.g., Hebei and Xinjiang Province) in China (Glawe 2004; Tai 1979; Zheng and Yu 1987; Zhuang 2005) and on Goji berry (L. barbarum) in China and Australia (Kiss et al. 2018; Wang et al. 2015). To our best knowledge, this is the first report of A. mougeotii on L. chinense in Henan Province, China. Because this powdery mildew could endanger L. chinense and reduce fruit production of this medicinal plant, disease control should be developed and followed.The author(s) declare no conflict of interest.References:Braun, U., and Cook, R. 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Metrics Article History Issue Date: 30 Oct 2020Published: 16 Sep 2020First Look: 27 May 2020Accepted: 24 May 2020 Pages: 3071-3071 Information© 2020 The American Phytopathological SocietyFundingFoundation for the Key Research Program of Higher Education of HenanGrant/Award Number: 20A210026Doctor Initiative Foundation of Henan Normal UniversityGrant/Award Number: 5101049170196Keywordspowdery mildewpathogen detectionArthrocladiella mougeotiiLycium chinenseinternal transcribed spacer (ITS)The author(s) declare no conflict of interest.PDF downloadCited byDeciphering the genome of Simplicillium aogashimaense to understand its mechanisms against the wheat powdery mildew fungus Blumeria graminis f. sp. tritici6 May 2022 | Phytopathology Research, Vol. 4, No. 1Biocontrol action of Trichothecium roseum against the wheat powdery mildew fungus Blumeria graminis f. sp. tritici15 November 2022 | Frontiers in Sustainable Food Systems, Vol. 6Podosphaera xanthii causing powdery mildew on Impatiens balsamina in China16 December 2021 | Canadian Journal of Plant Pathology, Vol. 44, No. 3First Report of Powdery Mildew Caused by Blumeria graminis f. sp. bromi on Bromus catharticus in ChinaMo Zhu, Jie Ji, Xiao Duan, Wenqi Shi, and Yong-Fang Li17 February 2021 | Plant Disease, Vol. 105, No. 4Occurrence of Powdery Mildew Caused by Blumeria graminis f. sp. poae on Poa pratensis in ChinaMo Zhu, Jie Ji, Wenqi Shi, and Yong-Fang Li17 February 2021 | Plant Disease, Vol. 105, No. 4
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