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BIBTEX RIS

Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence

2020; Nature Portfolio; Volume: 11; Issue: 1 Linguagem: Inglês

10.1038/s41467-020-16337-y

ISSN

2041-1723

Autores

Haruka Toda, Míriam Díaz-Varela, Joan Seguí-Barber, Wanlapa Roobsoong, Bàrbara Baró, Susana García‐Silva, Alicia Galiano, Melisa Gualdrón‐López, Anne Cristine Gomes de Almeida, Marcelo Augusto Mota Brito, Gisely Cardoso de Melo, Iris Aparici‐Herraiz, Carlos J. Castro-Cavadía, Wuelton Marcelo Monteiro, Eva Borràs, Eduard Sabidó, Igor C. Almeida, Jakub Chojnacki, Javier Martínez‐Picado, Marı́a Calvo, Pilar Armengol, Jaime Carmona‐Fonseca, María Fernanda Yasnot, Ricardo Lauzurica, Antonio Marcilla, Héctor Peinado, Mary R. Galinski, Marcus Lacerda, Jetsumon Sattabongkot, Carmen Fernández-Becerra, Hernando A. del Portillo,

Tópico(s)

Mosquito-borne diseases and control

Resumo

Abstract Plasmodium vivax is the most widely distributed human malaria parasite. Previous studies have shown that circulating microparticles during P. vivax acute attacks are indirectly associated with severity. Extracellular vesicles (EVs) are therefore major components of circulating plasma holding insights into pathological processes. Here, we demonstrate that plasma-derived EVs from Plasmodium vivax patients ( Pv EVs) are preferentially uptaken by human spleen fibroblasts ( h SFs) as compared to the uptake of EVs from healthy individuals. Moreover, this uptake induces specific upregulation of ICAM-1 associated with the translocation of NF-kB to the nucleus. After this uptake, P. vivax -infected reticulocytes obtained from patients show specific adhesion properties to h SFs, reversed by inhibiting NF-kB translocation to the nucleus. Together, these data provide physiological EV-based insights into the mechanisms of human malaria pathology and support the existence of P. vivax -adherent parasite subpopulations in the microvasculature of the human spleen.

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