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First Report of Pectobacterium aroidearum Causing Soft Rot of Pepper ( Capsicum annuum ) Fruits in Brazil

2020; American Phytopathological Society; Volume: 104; Issue: 11 Linguagem: Inglês

10.1094/pdis-02-20-0403-pdn

1943-7692

Alessandra Jackeline Guedes de Moraes, A. D. B. Baia, Elineide Barbosa de Souza, Ana Rosa Peixoto, Karol Alves Barroso, Carlos Almeida, Valdir de Queiroz Balbino, Will Jones Moura Soares da Silva, Marco Aurélio Siqueira da Gama,

Banana Cultivation and Research

HomePlant DiseaseVol. 104, No. 11First Report of Pectobacterium aroidearum Causing Soft Rot of Pepper (Capsicum annuum) Fruits in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Pectobacterium aroidearum Causing Soft Rot of Pepper (Capsicum annuum) Fruits in BrazilA. J. G. Moraes, A. D. B. Baia, E. B. Souza, A. R. Peixoto, K. A. Barroso, C. O. Almeida, V. Q. Balbino, W. J. Silva Jr., and M. A. S. GamaA. J. G. Moraeshttp://orcid.org/0000-0003-0960-3919Universidade Federal Rural da Amazônia, Belém, 66077-830, Pará, BrazilSearch for more papers by this author, A. D. B. BaiaUniversidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, BrazilSearch for more papers by this author, E. B. SouzaUniversidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, BrazilSearch for more papers by this author, A. R. PeixotoUniversidade do Estado da Bahia, Juazeiro, 48900-000, Bahia, BrazilSearch for more papers by this author, K. A. BarrosoUniversidade do Estado da Bahia, Juazeiro, 48900-000, Bahia, BrazilSearch for more papers by this author, C. O. AlmeidaUniversidade do Estado da Bahia, Juazeiro, 48900-000, Bahia, BrazilSearch for more papers by this author, V. Q. BalbinoUniversidade Federal de Pernambuco, Recife, 50670-901, Pernambuco, BrazilSearch for more papers by this author, W. J. Silva Jr.Universidade Federal de Pernambuco, Recife, 50670-901, Pernambuco, BrazilSearch for more papers by this author, and M. A. S. Gama†Corresponding author: M. A. S. Gama; E-mail Address: [email protected]http://orcid.org/0000-0001-6153-1700Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, BrazilSearch for more papers by this authorAffiliationsAuthors and Affiliations A. J. G. Moraes1 A. D. B. Baia2 E. B. Souza2 A. R. Peixoto3 K. A. Barroso3 C. O. Almeida3 V. Q. Balbino4 W. J. Silva Jr.4 M. A. S. Gama2 † 1Universidade Federal Rural da Amazônia, Belém, 66077-830, Pará, Brazil 2Universidade Federal Rural de Pernambuco, Recife, 52171-900, Pernambuco, Brazil 3Universidade do Estado da Bahia, Juazeiro, 48900-000, Bahia, Brazil 4Universidade Federal de Pernambuco, Recife, 50670-901, Pernambuco, Brazil Published Online:16 Sep 2020https://doi.org/10.1094/PDIS-02-20-0403-PDNAboutSectionsPDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat During field inspections carried out in August 2016, pepper (Capsicum annuum) plantations located in municipalities of the state of Pernambuco, Brazil, presented an incidence of 10 to 18% of pepper fruits still stuck to plants showing symptoms of soft rot. At least one pepper fruit with soft rot symptoms was collected at every municipality for identification of the bacterium. The strains were selectively isolated as described by Barroso et al. (2019). Fourteen strains were isolated, and each strain was obtained from a pepper fruit with soft rot symptoms. The strains CCRMPA106, CCRMPA109, CCRMPA131, and CCRMPA145 were obtained from Chã Grande; CCRMPA429 was obtained from Bezerros; and CCRMPA621, CCRMPA631, CCRMPA637, CCRMPA639, CCRMPA646, CCRMPA647, CCRMPA655, CCRMPA658, and CCRMPA670 were obtained from Petrolina. These strains were tested for pathogenicity in pepper fruits (cv. All Big). Ten microliters of bacterial suspension (107 CFU/ml) were deposited on wounds created with an entomological pin on the surface of pepper fruits. Six replicates were used for each isolate, with each repetition consisting of one pepper fruit. For negative and positive controls, pepper fruits were treated similarly with sterile distilled water and inoculated with one strain (CCRMPA35) of Pectobacterium aroidearum (Barroso et al. 2019). After 24 h of incubation at 28°C in a moist chamber, the soft rot symptoms were observed, and the strains were reisolated to complete Koch’s postulates. The negative control remained symptomless, whereas the positive control showed soft rot symptoms. The strains were identified using the Biolog Gen III System as Pectobacterium with a similarity level of 70%. Molecular identification was performed by sequencing a fragment of 1,400 bp of the 16S rRNA region (Hauben et al. 1998) and fragments of 450, 520, and 630 bp of the genes gapA, icdA, and proA (Ma et al. 2007). Using BLASTn analysis, the 16S rRNA region showed that the 14 strains shared 99 to 100% similarity with several species including P. aroidearum (SCRI109T). The Bayesian inference analysis (BIA) carried out using the sequences of the 16S rRNA region of the 14 strains (GenBank MK450555, MN883867, MK450557, MK450559, MK450563, MK450568, MK450572, MN883869, MK450573, MK450576, MK450577, MK450580, MK450582, and MN883868) and sequences of all species and subspecies of Pectobacterium, obtained from GenBank, grouped these strains with the strain SCRI109T, with a posterior probability above 90%. The strains CCRMPA109, CCRMPA637, and CCRMPA670 were selected for sequencing of the genes gapA (GenBank MK752922 to MK752924), icdA (GenBank MK752925 to MK752927), and proA (GenBank MK752928 to MK752930), and BLASTn analysis showed similarity values above 98% between these strains and SCRI109T. The BIA performed using the concatenated sequences of genes gapA, icdA, and proA of three strains together with the sequences of all species and subspecies of Pectobacterium, obtained from GenBank, grouped the three isolates with SCRI109T, with a posterior probability of 100%. P. aroidearum has been described as a plant pathogen with a preference for monocotyledonous plants (Nabhan et al. 2013). However, in recent years it has been observed causing soft rot in different dicotyledonous plants, such as zucchini (Moraes et al. 2017), lettuce, Chinese cabbage (Barroso et al. 2019) and, in the present study, pepper fruits. Thus, to our knowledge, this is the first report of P. aroidearum causing soft rot in pepper fruits in Brazil.The author(s) declare no conflict of interest.References:Barroso, K. A., et al. 2019. Plant Dis. 103:3274. https://doi.org/10.1094/PDIS-12-18-2237-PDN Link, ISI, Google ScholarHauben, L., et al. 1998. Syst. Appl. Microbiol. 21:384. https://doi.org/10.1016/S0723-2020(98)80048-9 Crossref, ISI, Google ScholarMa, B., et al. 2007. Phytopathology 97:1150. https://doi.org/10.1094/PHYTO-97-9-1150 Link, ISI, Google ScholarMoraes, A. J. G., et al. 2017. Plant Dis. 101:379. https://doi.org/10.1094/PDIS-08-16-1168-PDN Link, ISI, Google ScholarNabhan, S., et al. 2013. Int. J. Syst. Evol. Microbiol. 63:2520. https://doi.org/10.1099/ijs.0.046011-0 Crossref, ISI, Google ScholarThe author(s) declare no conflict of interest.Funding: Funding was provided by Conselho Nacional de Desenvolvimento Científico e Tecnológico (305843/2016-8).DetailsFiguresLiterature CitedRelated Vol. 104, No. 11 November 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionPlants of Echinacea purpurea affected by Verticillium dahliae (A. Garibaldi et al.). Photo credit: M. L. Gullino. Spinach plant infected with Stemphylium leaf spot (K. A. Spawton et al.). Photo credit: M. T. McGrath. Metrics Article History Issue Date: 30 Oct 2020Published: 16 Sep 2020First Look: 27 May 2020Accepted: 22 May 2020 Page: 3054 Information© 2020 The American Phytopathological SocietyFundingConselho Nacional de Desenvolvimento Científico e TecnológicoGrant/Award Number: 305843/2016-8Keywordsmacerative plant bacteriaBayesian inferencemonocotyledonous plantsdicotyledonous plantsThe author(s) declare no conflict of interest.PDF downloadCited byFirst Report of Pectobacterium aroidearum Causing Soft Rot in Olecranon Honey Peach (Prunus persica) in ChinaZhibin Liang, Huidi Liu, Zeling Xu, and Lian-Hui Zhang11 May 2022 | Plant Disease, Vol. 106, No. 6Analysis of the Taxonomy and Pathogenic Factors of Pectobacterium aroidearum L6 Using Whole-Genome Sequencing and Comparative Genomics2 July 2021 | Frontiers in Microbiology, Vol. 12