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Metal–Organic Framework Disintegrants: Enzyme Preparation Platforms with Boosted Activity

2020; Wiley; Volume: 59; Issue: 38 Linguagem: Inglês

10.1002/anie.202007827

1521-3773

Hongde An, Jie Song, Ting Wang, Nannan Xiao, Zhenjie Zhang, Peng Cheng, Shengqian Ma, He Huang, Yao Chen,

Advanced Nanomaterials in Catalysis

Abstract An enzyme formulation using customized enzyme activators (metal ions) to directly construct metal–organic frameworks (MOFs) as enzyme protective carriers is presented. These MOF carriers can also serve as the disintegrating agents to simultaneously release enzymes and their activators during biocatalysis with boosted activities. This highly efficient enzyme preparation combines enzyme immobilization (enhanced stability, easy operation) and homogeneous biocatalysis (fast diffusion, high activity). The MOF serves as an ion pump that continuously provides metal ion activators that greatly promote the enzymatic activities (up to 251 %). This MOF–enzyme composite demonstrated an excellent protective effect against various perturbation environments. A mechanistic investigation revealed that the spontaneous activator/enzyme release and ion pumping enable enzymes to sufficiently interact with their activators owing to the proximity effects, leading to a boost in biocatalytic performance.