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Revisado por pares
First Report of Stem Canker Caused by Neofusicoccum parvum and Pseudofusicoccum kimberleyense on Carya illinoinensis in Brazil
2020; American Phytopathological Society; Volume: 104; Issue: 11 Linguagem: Inglês
10.1094/pdis-01-20-0082-pdn
ISSN1943-7692
AutoresJéssica Mengue Rolim, Lucas Graciolli Savian, C. C. Walker, Jéssica Emília Rabuske, Janaína Silva Sarzi, Marlove Fátima Brião Muniz, Júlio Carlos Pereira da Silva,
Tópico(s)Banana Cultivation and Research
ResumoHomePlant DiseaseVol. 104, No. 11First Report of Stem Canker Caused by Neofusicoccum parvum and Pseudofusicoccum kimberleyense on Carya illinoinensis in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Stem Canker Caused by Neofusicoccum parvum and Pseudofusicoccum kimberleyense on Carya illinoinensis in BrazilJ. M. Rolim, L. G. Savian, C. Walker, J. E. Rabuske, J. S. Sarzi, M. F. B. Muniz, and J. C. P. da SilvaJ. M. Rolim†Corresponding author: J. M. Rolim; E-mail Address: [email protected]http://orcid.org/0000-0003-2737-7599Universidade Federal de Pelotas, Departamento de Fitotecnia, Pelotas, BrazilSearch for more papers by this author, L. G. Savianhttp://orcid.org/0000-0002-4398-7998Universidade Federal de Santa Maria, Departamento de Defesa Fitossanitária, Santa Maria, BrazilSearch for more papers by this author, C. WalkerUniversidade Federal de Santa Maria, Departamento de Defesa Fitossanitária, Santa Maria, BrazilSearch for more papers by this author, J. E. RabuskeUniversidade Federal de Santa Maria, Departamento de Defesa Fitossanitária, Santa Maria, BrazilSearch for more papers by this author, J. S. SarziUniversidade Federal de Santa Maria, Departamento de Defesa Fitossanitária, Santa Maria, BrazilSearch for more papers by this author, M. F. B. MunizUniversidade Federal de Santa Maria, Departamento de Defesa Fitossanitária, Santa Maria, BrazilSearch for more papers by this author, and J. C. P. da SilvaUniversidade Federal de Santa Maria, Departamento de Defesa Fitossanitária, Santa Maria, BrazilSearch for more papers by this authorAffiliationsAuthors and Affiliations J. M. Rolim1 † L. G. Savian2 C. Walker2 J. E. Rabuske2 J. S. Sarzi2 M. F. B. Muniz2 J. C. P. da Silva2 1Universidade Federal de Pelotas, Departamento de Fitotecnia, Pelotas, Brazil 2Universidade Federal de Santa Maria, Departamento de Defesa Fitossanitária, Santa Maria, Brazil Published Online:16 Sep 2020https://doi.org/10.1094/PDIS-01-20-0082-PDNAboutSectionsView articlePDFSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articlePecan (Carya illinoinensis [Wangenh.] K. Koch) belongs to the Juglandaceae family and is an economically valuable crop in southern Brazil. For years, cankers have been observed in pecan orchards in the country. However, there are no reports of organisms responsible for cankers on pecan in Brazil. Therefore, in 2017 we collected trunk samples from 5-year-old pecan cultivar Barton trees with cankers from orchards in the municipalities of Santa Maria and Pantano Grande, Rio Grande do Sul, Brazil, to determine their possible biotic cause. The incidence was about 60% in the two sampled orchards. The symptomatic trees presented lengthwise cracking, tissues with dark necrosis, and formation of cankers along the trunk. Five fragments were collected from on one tree from each orchard by cutting with a blade. The samples were surface sterilized with 70% ethanol for 5 min, followed by 1% NaOCl for 2 min, and then washed three times in sterile water. The fragments were transferred to germination boxes and incubated at 25 ± 2°C with 12 h of light. After 3 days, two similar mycelial growth patterns were observed in 80% of the fragments. The fungi were transferred to potato dextrose agar (PDA) for 7 days at 25 ± 2°C and then to pine needle agar (PNA) at 25 ± 2°C for 20 days to stimulate the production of reproductive structures (Lima et al. 2013). From PDA, two pure isolates, SM21 (from Santa Maria) and PAN1 (from Pantano Grande), were obtained by hyphal-tip transference. The two isolates used in the study were representative of the fungi that grew in most fragments; colonies showed olive dark green and grayish olive coloration, respectively. Both fungal cultures produced an average diameter of 90 mm of mycelial growth after 7 days on PDA at 25 ± 2°C. On PNA, both fungi produced pycnidia covered by gray aerial mycelium, but no spores were found after 45 days. DNA from each isolate was extracted according to the method of Leslie and Summerell (2006), and the elongation factor 1-α region was sequenced using the primers EF1-728F and EF1-928R (Carbone and Kohn 1999). Phylogenetic analysis was performed using the neighbor-joining method with 1,000 replicates (software Mega 4.0). The sequences obtained were deposited in GenBank (SM21, MN233653; PAN1, MN258734) and showed, 99.24% genetic identity with Neofusicoccum parvum (MN175952.1) and 99.27% with Pseudofusicoccum kimberleyense (EU144071.1). The fungal cultures were deposited in the collection of the Herbarium SMDB of the Universidade Federal de Santa Maria under registration numbers 18364 and 18635 for SM21 and PAN1, respectively. The pathogenicity was evaluated on 3-month-old pecan seedlings (cv. Barton) in a greenhouse. PDA discs with mycelium of each isolate (14 days old) were used to inoculate the seedlings by introducing one disc into a 3-mm-diameter hole made in each stem, 12 cm above the neck. For the control plants, noninoculated PDA discs were used. Four seedlings were used per treatment. After 40 days, all inoculated plants initially showed lengthwise cracking followed by tissue necrosis and cankers similar to those observed in the orchards. The control plants remained symptom-free. Subsequently, the fungi were reisolated from symptomatic plants, and the morphological characteristics, previously described on PDA, were the same, fulfilling Koch’s postulates. The fungi were not reisolated from control stems. Even though N. parvum and P. kimberleyense have already been related to cankers in other countries in different cultures (Luo et al. 2019; Sakalidis et al. 2011), this is the first report of both species causing stem canker on pecan in Brazil.The author(s) declare no conflict of interest.References:Carbone, I., and Kohn, L. M. 1999. Mycologia 91:553. Google ScholarLeslie, J. F. and Summerell, B. A. 2006. The Fusarium Laboratory Manual. Blackwell Publishing, Ames, IA. doi.org/10.1002/9780470278376 Google ScholarLima, J. S., et al. 2013. Summa Phytopathol. 39:81. https://doi.org/10.1590/S0100-54052013000200001 Crossref, Google ScholarLuo, Y., et al. 2019. Plant Dis. 103:2374. https://doi.org/10.1094/PDIS-11-18-1963-RE Link, ISI, Google ScholarSakalidis, M. L., et al. 2011. Fungal Ecol. 4:1. https://doi.org/10.1016/j.funeco.2010.06.001 Crossref, ISI, Google ScholarL. G. Savian, C. Walker, J. E. Rabuske, J. S. Sarzi, M. F. B. Muniz, and J. C. P. da Silva contributed equally.The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 104, No. 11 November 2020SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionPlants of Echinacea purpurea affected by Verticillium dahliae (A. Garibaldi et al.). Photo credit: M. L. Gullino. Spinach plant infected with Stemphylium leaf spot (K. A. Spawton et al.). Photo credit: M. T. McGrath. Metrics Article History Issue Date: 30 Oct 2020Published: 16 Sep 2020First Look: 3 Jun 2020Accepted: 30 May 2020 Page: 3067 Information© 2020 The American Phytopathological SocietyKeywordspecan nuttreepathogenicityThe author(s) declare no conflict of interest.PDF downloadCited byPseudofusicoccum kimberleyenseCABI Compendium, Vol. CABI CompendiumNeofusicoccum parvumCABI Compendium, Vol. CABI CompendiumFUNGI OF THE BOTRYOSPHAERIACEAE FAMILY CAUSE DIFFERENT LEVELS OF STEM CANKER ON PECAN TREES (Carya illinoinensis) IN BRAZIL1 January 2022 | Revista Árvore, Vol. 46Pseudofusicoccum sp. causing shoot canker in peach in Uruguay27 February 2021 | Australasian Plant Disease Notes, Vol. 16, No. 1
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