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HEAVY METAL RESISTANCE AND METALLOTHIONEIN INDUCTION IN BACTERIA ISOLATED FROM SEYBOUSE RIVER, ALGERIA

2020; Hungarian University of Agriculture and Life Sciences; Volume: 18; Issue: 1 Linguagem: Inglês

10.15666/aeer/1801_17211737

1785-0037

Lamia Benhalima, Sirine Amri, Mourad Bensouilah, R. Ouzrout,

Heavy metals in environment

Heavy metal pollution is a serious and widespread environmental problem that destroys microbial ecology.In this study, determination of copper and cadmium concentrations and heavy metal-resistant bacteria identification in water samples obtained from four sites at Seybouse River in Algeria were carried out.The minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) were determined in culture media with 12.5-3600 µg/mL of cadmium and copper salts.Metallothionein (MT) production was evaluated by spectrophotometry methodology.Trace metal element concentrations determined in the water samples revealed spatial variations for Cu and Cd.A total of 12 copper-and cadmium-resistant bacteria (KZ1-KZ12) were isolated from surface water in Seybouse River.Five isolates KZ2, KZ5, KZ8, KZ10 and KZ11 showed high values of minimum inhibitory concentration (MIC = 3600 µg/mL) for each heavy metal.High MBC of the strain toward Cu and Cd was found to be >3600 µg/mL.The MT concentration in five best isolates which showed high resistances increased with increasing metal concentration (r = 0.936, p = 0.006).High levels of MT are detected in bacterial strains exposed to Cd (51.66-90.53nmol MT/g bacterial biomass).These indicated that the isolates can be used efficiently in removal of heavy metals in contaminated sites.